• Western blot analysis of lysates from Rat heart, using AFM Rabbit polyclonal antibody (STJ117448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1S.

Anti-AFM antibody (260-599) (STJ117448)

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STJ117448

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AFM (260-599) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AFM
Gene ID: 173
Uniprot ID: AFAM_HUMAN
Immunogen Region: 260-599
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-599 of human AFM (NP_001124.1).
Immunogen Sequence: EDVSSNYDGCCEGDVVQCIR DTSKVMNHICSKQDSISSKI KECCEKKIPERGQCIINSNK DDRPKDLSLREGKFTDSENV CQERDADPDTFFAKFTFEYS RRHPDLSIPELLRIVQIYKD LLRNCCNTENPPGCYRYAED KFNETTEKSLKMVQQECKHF QNLGKDGLKYHYLIRLTKIA PQLSTEELVSLGEKMVTAFT TCCTLSEEFACVDNLADLVF GELCGVNENRTINPAVDHC
Tissue Specificity High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level).
Post Translational Modifications N-glycosylated.more than 90% of the glycans are sialylated.
Function Functions as carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.
Protein Name Afamin
Alpha-Albumin
Alpha-Alb
Cellular Localisation Secreted
Alternative Antibody Names Anti-Afamin antibody
Anti-Alpha-Albumin antibody
Anti-Alpha-Alb antibody
Anti-AFM antibody
Anti-ALB2 antibody
Anti-ALBA antibody

Information sourced from Uniprot.org

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