| Short Description : | Rabbit polyclonal AEBP2 (234-503) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | AEBP2 |
| Gene ID: | 121536 |
| Uniprot ID: | AEBP2_HUMAN |
| Immunogen Region: | 234-503 |
| Immunogen Sequence: | STISSGRSTPAMMNGQGSTT SSSKNIAYNCCWDQCQACFN SSPDLADHIRSIHVDGQRGG VFVCLWKGCKVYNTPSTSQS WLQRHMLTHSGDKPFKCVVG GCNASFASQGGLARHVPTHF SQQNSSKVSSQPKAKEESPS KAGMNKRRKLKNKRRRSLPR PHDFFDAQTLDAIRHRAICF NLSAHIESLGKGHSVVFHST VIAKRKEDSGKIKLLLHWMP EDILPDVWVNESERHQLKT |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human AEBP2 (NP_694939.2). |
| Function | Acts as an accessory subunit for the core Polycomb repressive complex 2 (PRC2), which mediates histone H3K27 (H3K27me3) trimethylation on chromatin leading to transcriptional repression of the affected target gene. Plays a role in nucleosome localization of the PRC2 complex. |
| Protein Name | Zinc Finger Protein Aebp2Adipocyte Enhancer-Binding Protein 2Ae-Binding Protein 2 |
| Database Links | Reactome: R-HSA-212300Reactome: R-HSA-3214841 |
| Cellular Localisation | NucleusLocalizes To Chromatin As Part Of The Prc2 Complex |
| Alternative Antibody Names | Anti-Zinc Finger Protein Aebp2 antibodyAnti-Adipocyte Enhancer-Binding Protein 2 antibodyAnti-Ae-Binding Protein 2 antibodyAnti-AEBP2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (STJ119228) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98100/410505/STJ119228_1__00390.1744896984.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (STJ119228) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
