Anti-ADAMTS13 antibody (50-300) (STJ110780)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ADAMTS13 (50-300) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ADAMTS13 |
| Gene ID | 11093 |
| Uniprot ID | ATS13_HUMAN |
| Immunogen Region | 50-300 |
| Immunogen Sequence | LSPGAPLKGRPPSPGFQRQR QRQRRAAGGILHLELLVAVG PDVFQAHQEDTERYVLTNLN IGAELLRDPSLGAQFRVHLV KMVILTEPEGAPNITANLTS SLLSVCGWSQTINPEDDTDP GHADLVLYITRFDLELPDGN RQVRGVTQLGGACSPTWSCL ITEDTGFDLGVTIAHEIGHS FGLEHDGAPGSGCGPSGHVM ASDGAAPRAGLAWSPCSRRQ LLSLLSAGRARCVWDPPRP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human ADAMTS13 (NP_620594.1). |
Additional Info
| Tissue Specificity | Plasma. Expressed primarily in liver. |
| Post Translational Modifications | Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS13. May also be C-glycosylated on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and also N-glycosylated. These other glycosylations can also facilitate secretion. The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. |
| Function | Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. |
| Protein Name | A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13Adam-Ts 13Adam-Ts13Adamts-13Von Willebrand Factor-Cleaving ProteaseVwf-CpVwf-Cleaving Protease |
| Database Links | Reactome: R-HSA-5083635Reactome: R-HSA-5173214Reactome: R-HSA-75892Reactome: R-HSA-9845619Reactome: R-HSA-9845621 |
| Cellular Localisation | SecretedSecretion Enhanced By O-Fucosylation Of Tsp Type-1 Repeats |
| Alternative Antibody Names | Anti-A Disintegrin And Metalloproteinase With Thrombospondin Motifs 13 antibodyAnti-Adam-Ts 13 antibodyAnti-Adam-Ts13 antibodyAnti-Adamts-13 antibodyAnti-Von Willebrand Factor-Cleaving Protease antibodyAnti-Vwf-Cp antibodyAnti-Vwf-Cleaving Protease antibodyAnti-ADAMTS13 antibodyAnti-C9orf8 antibodyAnti-UNQ6102 antibodyAnti-PRO20085 antibody |
Information sourced from Uniprot.org