• Immunofluorescence analysis of HeLa cells using ADAM10 antibody (STJ112469) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of RAW264. 7, using ADAM10 antibody (STJ112469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.

Anti-ADAM10 antibody (218-433) (STJ112469)

SKU:
STJ112469

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADAM10 (218-433) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADAM10
Gene ID: 102
Uniprot ID: ADA10_HUMAN
Immunogen Region: 218-433
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 218-433 of human ADAM10 (NP_001101.1).
Immunogen Sequence: EKNTCQLYIQTDHLFFKYYG TREAVIAQISSHVKAIDTIY QTTDFSGIRNISFMVKRIRI NTTADEKDPTNPFRFPNIGV EKFLELNSEQNHDDYCLAYV FTDRDFDDGVLGLAWVGAPS GSSGGICEKSKLYSDGKKKS LNTGIITVQNYGSHVPPKVS HITFAHEVGHNFGSPHDSGT ECTPGESKNLGQKENGNYIM YARATSGDKLNNNKFS
Tissue Specificity Expressed in the brain (at protein level). Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
Post Translational Modifications The precursor is cleaved by furin and PCSK7.
Function Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3. Mediates the proteolytic cleavage of IL6R and IL11RA, leading to the release of secreted forms of IL6R and IL11RA. Enhances the cleavage of CHL1 by BACE1. Cleaves NRCAM. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain. Involved in the development and maturation of glomerular and coronary vasculature. During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions. May regulate the EFNA5-EPHA3 signaling. (Microbial infection) Promotes the cytotoxic activity of S.aureus hly by binding to the toxin at zonula adherens and promoting formation of toxin pores.
Protein Name Disintegrin And Metalloproteinase Domain-Containing Protein 10
Adam 10
Cdw156
Kuzbanian Protein Homolog
Mammalian Disintegrin-Metalloprotease
Cd Antigen Cd156c
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-177929
Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2660826
Reactome: R-HSA-2691232
Reactome: R-HSA-2894862
Reactome: R-HSA-2979096
Reactome: R-HSA-381426
Reactome: R-HSA-3928665
Reactome: R-HSA-6798695
Reactome: R-HSA-8957275
Reactome: R-HSA-9013507
Reactome: R-HSA-9013700
Reactome: R-HSA-977225
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus Membrane
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
Cell Projection
Axon
Dendrite
Cell Junction
Adherens Junction
Cytoplasm
Is Localized In The Plasma Membrane But Is Also Expressed In The Golgi Apparatus And In Clathrin-Coated Vesicles Derived Likely From The Golgi
During Long Term Depression
It Is Recruited To The Cell Membrane By Dlg1
The Immature Form Is Mainly Located Near Cytoplasmic Fibrillar Structures
While The Mature Form Is Predominantly Located At Zonula Adherens And The Cell Membrane
The Localization And Clustering Of Mature Adam10 To Zonula Adherens Is Regulated By Afdn
Tspan33
Plekha7 And Pdzd11
Alternative Antibody Names Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 10 antibody
Anti-Adam 10 antibody
Anti-Cdw156 antibody
Anti-Kuzbanian Protein Homolog antibody
Anti-Mammalian Disintegrin-Metalloprotease antibody
Anti-Cd Antigen Cd156c antibody
Anti-ADAM10 antibody
Anti-KUZ antibody
Anti-MADM antibody

Information sourced from Uniprot.org

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