• Immunofluorescence analysis of mouse bone marrow cells using ACTN3 Rabbit monoclonal antibody (STJ11103061) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat bone marrow cells using ACTN3 Rabbit monoclonal antibody (STJ11103061) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ACTN3 Rabbit monoclonal antibody (STJ11103061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACTN3 antibody (700-800) [S1MR] (STJ11103061)

SKU:
STJ11103061

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ACTN3 (700-800) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN3
Gene ID: 89
Uniprot ID: ACTN3_HUMAN
Immunogen Region: 700-800
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 700-800 of human ACTN3 (Q08043).
Immunogen Sequence: DRLEGDHQLLQESLVFDNKH TVYSMEHIRVGWEQLLTSIA RTINEVENQVLTRDAKGLSQ EQLNEFRASFNHFDRKQNGM MEPDDFRACLISMGYDLGEV E
Tissue Specificity Expressed only in a subset of type 2 skeletal muscle fibers.
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name Alpha-Actinin-3
Alpha-Actinin Skeletal Muscle Isoform 3
F-Actin Cross-Linking Protein
Database Links Reactome: R-HSA-373753
Reactome: R-HSA-390522
Alternative Antibody Names Anti-Alpha-Actinin-3 antibody
Anti-Alpha-Actinin Skeletal Muscle Isoform 3 antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-ACTN3 antibody

Information sourced from Uniprot.org

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