Anti-Activity-regulated gene 3.1 protein homolog antibody (STJA0040978)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the C terminal of human Arg3.1 protein (ARC protein) conjugated to Blue Carrier Protein. The sequence is homologous with mouse and rat form of Arg3.1.
General Information
| Short Description | Rabbit polyclonal anti-Activity-regulated gene 3.1 protein homolog for use in IHC-F in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | IHC 2-4 µg/mL |
| Formulation | Lyophilized from a solution containing PBS pH 7.4 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacteri |
Target Information
| Gene Symbol | ARC |
| Gene ID | 23237 |
| Uniprot ID | ARC_HUMAN |
| Immunogen | A synthetic peptide from the C terminal of human Arg3.1 protein (ARC protein) conjugated to Blue Carrier Protein. The sequence is homologous with mouse and rat form of Arg3.1. |
| Immunogen Sequence | Human |
| Specificity | Specific for Arg3.1. Rat and mouse. Other species not yet tested. |
Additional Info
| Post Translational Modifications | Palmitoylation anchors the protein into the membrane by allowing direct insertion into the hydrophobic core of the lipid bilayer. Ubiquitinated by UBE3A, leading to its degradation by the proteasome, thereby promoting AMPA receptors (AMPARs) expression at synapses. Ubiquitinated by RNF216 at Lys-268 and Lys-269 limiting ARC protein levels induced by synaptic activity and thus regulating ARC-dependent forms of synaptic plasticity. Phosphorylation at Ser-260 by CaMK2 prevents homooligomerization into virion-like capsids by disrupting an interaction surface essential for high-order oligomerization. Phosphorylation by CaMK2 inhibits synaptic activity. |
| Function | Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory. Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1. In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation. |
| Protein Name | Activity-Regulated Cytoskeleton-Associated ProteinHarcActivity-Regulated Gene 3.1 Protein HomologArc/Arg3.1Arg3.1 |
| Database Links | Reactome: R-HSA-9031628 |
| Cellular Localisation | Extracellular Vesicle MembraneLipid-AnchorPostsynaptic Cell MembraneSynapsePostsynaptic DensityEarly Endosome MembraneCell ProjectionDendriteCytoplasmCytoskeletonCell CortexDendritic SpineCytoplasmic VesicleSecretory VesicleAcrosomeClathrin-Coated Vesicle MembraneForms Virion-Like Extracellular Vesicles That Are Released From NeuronsEnriched In Postsynaptic Density Of Dendritic SpinesTargeted To Inactive Synapses Following Interaction With Camk2b In The Kinase Inactive StateAccumulation At Weaker Synapses May Be Required To Prevent Their Undesired EnhancementAssociated With The Cell Cortex Of Neuronal Soma And DendritesAssociated With The Sperm Tail |
| Alternative Antibody Names | Anti-Activity-Regulated Cytoskeleton-Associated Protein antibodyAnti-Harc antibodyAnti-Activity-Regulated Gene 3.1 Protein Homolog antibodyAnti-Arc/Arg3.1 antibodyAnti-Arg3.1 antibodyAnti-ARC antibodyAnti-KIAA0278 antibody |
Information sourced from Uniprot.org