Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (STJ11107554). <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-Actin-Plant Specific antibody [SMM5489] (STJ11107554)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
STJ11107554
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General Information

Short DescriptionMouse monoclonal Actin-Plant Specific antibody for use in WB and ELISA in various plants samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostMouse
ReactivityVarious Plants
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSMM5489
IsotypeIgG1
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:2000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 1% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

SpecificityA synthetic peptide corresponding to Actin (plant specific).

Additional Info

Background Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Information sourced from Uniprot.org

Citations

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