• Western blot analysis of extracts of HepG2 cells, using ACSS2 Rabbit monoclonal antibody (STJ11101408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using ACSS2 Rabbit monoclonal antibody (STJ11101408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ACSS2 antibody (50-150) [S8MR] (STJ11101408)

SKU:
STJ11101408

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSS2
Gene ID: 55902
Uniprot ID: ACSA_HUMAN
Immunogen Region: 50-150
Specificity: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ACSS2 (Q9NR19).
Immunogen Sequence: LHRRSVEEPREFWGDIAKEF YWKTPCPGPFLRYNFDVTKG KIFIEWMKGATTNICYNVLD RNVHEKKLGDKVAFYWEGNE PGETTQITYHQLLVQVCQFS N
Post Translational Modifications Reversibly acetylated at Lys-661. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3. Glucose deprivation results in its AMPK-dependent phosphorylation at Ser-659, which leads to exposure of its nuclear localization signal, required for its interaction with KPNA1 and subsequent translocation to the nucleus.
Function Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity. Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis. Glucose deprivation results in AMPK-mediated phosphorylation of ACSS2 leading to its translocation to the nucleus where it binds to TFEB and locally produces acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes thereby activating their transcription. The regulation of genes associated with autophagy and lysosomal activity through ACSS2 is important for brain tumorigenesis and tumor survival. Acts as a chromatin-bound transcriptional coactivator that up-regulates histone acetylation and expression of neuronal genes. Can be recruited to the loci of memory-related neuronal genes to maintain a local acetyl-CoA pool, providing the substrate for histone acetylation and promoting the expression of specific genes, which is essential for maintaining long-term spatial memory.
Protein Name Acetyl-Coenzyme A Synthetase - Cytoplasmic
Acetate--Coa Ligase
Acetyl-Coa Synthetase
Acs
Acecs
Acetyl-Coa Synthetase 1
Acecs1
Acyl-Coa Synthetase Short-Chain Family Member 2
Acyl-Activating Enzyme
Propionate--Coa Ligase
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Glucose Deprivation Results In Its Ampk-Dependent Phosphorylation And Subsequent Nuclear Translocation
Phosphorylation At Ser-659
Leads To Exposure Of Its Nuclear Localization Signal Which Is Required For Its Interaction With Kpna1 And Subsequent Translocation To The Nucleus
Found In The Cytoplasm In Undifferentiated Neurons And Upon Differentiation
Translocates To Nucleus
Alternative Antibody Names Anti-Acetyl-Coenzyme A Synthetase - Cytoplasmic antibody
Anti-Acetate--Coa Ligase antibody
Anti-Acetyl-Coa Synthetase antibody
Anti-Acs antibody
Anti-Acecs antibody
Anti-Acetyl-Coa Synthetase 1 antibody
Anti-Acecs1 antibody
Anti-Acyl-Coa Synthetase Short-Chain Family Member 2 antibody
Anti-Acyl-Activating Enzyme antibody
Anti-Propionate--Coa Ligase antibody
Anti-ACSS2 antibody
Anti-ACAS2 antibody

Information sourced from Uniprot.org

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