• Western blot analysis of extracts of Rat stomach, using MICA antibody (STJ11102988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using MICA antibody (STJ11102988) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MICA antibody (50-150) [S8MR] (STJ11102988)

SKU:
STJ11102988

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MICA (50-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MICA
Gene ID: 100507436
Uniprot ID: MICA_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MICA (Q29983).
Immunogen Sequence: HLDGQPFLRCDRQKCRAKPQ GQWAEDVLGNKTWDRETRDL TGNGKDLRMTLAHIKDQKEG LHSLQEIRVCEIHEDNSTRS SQHFYYDGELFLSQNLETKE W
Tissue Specificity Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells.
Post Translational Modifications N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
Function Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Protein Name Mhc Class I Polypeptide-Related Sequence A
Mic-A
Database Links Reactome: R-HSA-198933
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Expressed On The Cell Surface In Gastric Epithelium
Endothelial Cells And Fibroblasts And In The Cytoplasm In Keratinocytes And Monocytes
Infection With Human Adenovirus 5 Suppresses Cell Surface Expression Due To The Adenoviral E3-19k Protein Which Causes Retention In The Endoplasmic Reticulum
Alternative Antibody Names Anti-Mhc Class I Polypeptide-Related Sequence A antibody
Anti-Mic-A antibody
Anti-MICA antibody
Anti-PERB11.1 antibody

Information sourced from Uniprot.org

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