• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody (STJ117427) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ117427) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-Acetyl-Histone H4-K5 antibody (STJ117427)

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STJ117427
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Host: Rabbit
Applications: WB, IHC, IF, ChIP
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal primary antibody against Histone H4.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
ChIP: 1:20-1:100
Immunogen: A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
1 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Information sourced from uniprot.org

6 months standard