• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody (STJ29395) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A17559) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A17559) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A17559) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (STJ29395) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat heart using Acetyl-Histone H3-K27 antibody (STJ29395) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human breast using Acetyl-Histone H3-K27 antibody (STJ29395) at dilution of 1:200 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (STJ29395) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-Acetyl-Histone H3-K27 antibody (STJ29395)

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STJ29395
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Host: Rabbit
Applications: WB, IHC, IF, IP, ChIP, ChIPseq
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal primary antibody against Histone H3.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
ChIP: 1:20-1:100
ChIPseq: 1:20-1:100
Uniprot ID: H31T_HUMAN
Gene ID: 8290
Gene Symbol: H3-4
Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Tissue Specificity Expressed in testicular cells.
Database Links Reactome: R-HSA-110328
Cellular Localisation Nucleus
Chromosome
Protein Name Histone H3.1t
H3/T
H3t
H3/G
Histone H3.4
Alternative Names Anti-Histone H3.1t antibody
Anti-H3/T antibody
Anti-H3t antibody
Anti-H3/G antibody
Anti-Histone H3.4 antibody
Anti-H3-4 antibody
Anti-H3FT antibody
Anti-HIST3H3 antibody

Information sourced from uniprot.org

6 months standard