• Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K14 Rabbit polyclonal antibody (STJ29396) at dilution of 1:100 (40x lens). U-2 OS cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K14 Rabbit polyclonal antibody (STJ29396) at 1:400 dilution. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
  • Western blot analysis of lysates from NIH/3T3 cells using Acetyl-Histone H3-K14 Rabbit polyclonal antibody (STJ29396) at 1:400 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.
  • Western blot analysis of lysates from C6 cells using Acetyl-Histone H3-K14 Rabbit polyclonal antibody (STJ29396) at 1:400 dilution. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-Acetyl-Histone H3-K14 antibody (STJ29396)

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STJ29396

$174.83 - $863.33
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H3-K14 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic acetylated peptide around K14 of human H3 (NP_003520.1).
Immunogen Sequence: GGKAP
Background The protein encoded by this gene is a membrane protein found at the tight junctions between epithelial cells. The encoded protein helps establish epithelial barriers such as those in the organ of Corti, where these barriers are required for normal hearing. Defects in this gene are a cause of deafness autosomal recessive type 49 (DFNB49). Two transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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