• Chromatin immunoprecipitation was performed with cross-linked chromatin from 293F cells, using Acetyl-Histone H2B-K20 Rabbit polyclonal antibody (STJ11105525) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.
  • Western blot analysis of various lysates using Acetyl-Histone H2B-K20 Rabbit polyclonal antibody (STJ11105525) at 1:500 dilution. HeLa cells, NIH/3T3 cells and C6 cells were treated by TSA (1 Mu M) at 37 °C for 18 hours. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 20s.

Anti-Acetyl-Histone H2B-K20 antibody (STJ11105525)

SKU:
STJ11105525

£129.50 - £642.50
Current Stock:
Host: Rabbit
Applications: WB/ELISA/ChIP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
ChIP:5 Mu g antibody for 5 Mu g-10 Mu g of Chromatin
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H2BC21
H2BC4.H2BC6.H2BC7.H2BC8.H2BC10
Gene ID: 8349
3017/8339/8343/8344/8346/8347
Uniprot ID: H2B2E_HUMAN
H2B1C_HUMAN
Specificity: A synthetic acetylated peptide around K20 of human Histone H2B (NP_003519.1).
Tissue Specificity
Alternative Antibody Names Anti-Histone H2b Type 2-E antibody
Anti-H2b-Clustered Histone 21 antibody
Anti-Histone H2b-Gl105 antibody
Anti-Histone H2b.q antibody
Anti-H2b/Q antibody
Anti-H2BC21 antibody
Anti-H2BFQ antibody
Anti-HIST2H2BE antibody
Anti-Histone H2b Type 1-C/E/F/G/I antibody
Anti-Histone H2b.1 A antibody
Anti-Histone H2b.a antibody
Anti-H2b/A antibody
Anti-Histone H2b.g antibody
Anti-H2b/G antibody
Anti-Histone H2b.h antibody
Anti-H2b/H antibody
Anti-Histone H2b.k antibody
Anti-H2b/K antibody
Anti-Histone H2b.l antibody
Anti-H2b/L antibody
Anti-H2BC4 antibody
Anti-H2BFL antibody
Anti-HIST1H2BC.H2BC6 antibody
Anti-H2BFH antibody
Anti-HIST1H2BE.H2BC7 antibody
Anti-H2BFG antibody
Anti-HIST1H2BF.H2BC8 antibody
Anti-H2BFA antibody
Anti-HIST1H2BG.H2BC10 antibody
Anti-H2BFK antibody
Anti-HIST1H2BI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance