• Western blot analysis of extracts of various cell lines, using ACC1 antibody (STJ118055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using ACC1 rabbit polyclonal antibody (STJ118055) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using ACC1 rabbit polyclonal antibody (STJ118055) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using ACC1 rabbit polyclonal antibody (STJ118055) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ACC1 antibody (STJ118055). Western blot was performed from the immunoprecipitate using ACC1 antibody (STJ118055) at a dilition of 1:1000.

Anti-Acetyl-ACACA antibody (STJ118055)

SKU:
STJ118055

£166.50 - £446.50
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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Acetyl-CoA carboxylase alpha is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: ACACA
Gene ID: 31
Uniprot ID: ACACA_HUMAN
Immunogen: A synthetic peptide of human ACC1.
Tissue Specificity Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues.expressed at low level in pulmonary tissue.not detected in the liver.
Post Translational Modifications Phosphorylation on Ser-1263 is required for interaction with BRCA1. Phosphorylation at Ser-80 by AMPK inactivates enzyme activity. The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity.
Function Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.
Protein Name Acetyl-Coa Carboxylase 1
Acc1
Acetyl-Coenzyme A Carboxylase Alpha
Acc-Alpha
Database Links Reactome: R-HSA-163765
Reactome: R-HSA-196780
Reactome: R-HSA-200425
Reactome: R-HSA-2426168
Reactome: R-HSA-3371599
Reactome: R-HSA-75105
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Acetyl-Coa Carboxylase 1 antibody
Anti-Acc1 antibody
Anti-Acetyl-Coenzyme A Carboxylase Alpha antibody
Anti-Acc-Alpha antibody
Anti-ACACA antibody
Anti-ACAC antibody
Anti-ACC1 antibody
Anti-ACCA antibody

Information sourced from Uniprot.org

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