• Western blot analysis of the lysates from HT-29 cells using PHCA antibody.
  • Western blot analysis of lysates from COLO cells, using PHCA Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of MCF7 cells, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using PHCA Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using APHC Polyclonal Antibody

Anti-ACER3 antibody (171-220 aa) (STJ91628)

SKU:
STJ91628

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Alkaline ceramidase 3 (171-220 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACER3
Gene ID: 55331
Uniprot ID: ACER3_HUMAN
Immunogen Region: 171-220 aa
Specificity: APHC Polyclonal Antibody detects endogenous levels of APHC protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PHCA at the amino acid range 171-220
Function Endoplasmic reticulum and Golgi ceramidase that catalyzes the hydrolysis of unsaturated long-chain C18:1-, C20:1- and C20:4-ceramides, dihydroceramides and phytoceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Controls the generation of sphingosine in erythrocytes, and thereby sphingosine-1-phosphate in plasma. Through the regulation of ceramides and sphingosine-1-phosphate homeostasis in the brain may play a role in neurons survival and function. By regulating the levels of pro-inflammatory ceramides in immune cells and tissues, may modulate the inflammatory response.
Protein Name Alkaline Ceramidase 3
Alkcdase 3
Alkaline Cdase 3
Alkaline Dihydroceramidase Sb89
Alkaline Phytoceramidase
Aphc
Database Links Reactome: R-HSA-1660661
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Golgi Apparatus Membrane
Alternative Antibody Names Anti-Alkaline Ceramidase 3 antibody
Anti-Alkcdase 3 antibody
Anti-Alkaline Cdase 3 antibody
Anti-Alkaline Dihydroceramidase Sb89 antibody
Anti-Alkaline Phytoceramidase antibody
Anti-Aphc antibody
Anti-ACER3 antibody
Anti-APHC antibody
Anti-PHCA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance