• Immunohistochemistry analysis of paraffin-embedded mouse kidney using ACAT1 antibody (STJ115238) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using ACAT1 antibody (STJ115238) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using ACAT1 antibody (STJ115238) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using ACAT1 antibody (STJ115238) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ACAT1 antibody (1-145) (STJ115238)

SKU:
STJ115238

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACAT1 (1-145) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACAT1
Gene ID: 38
Uniprot ID: THIL_HUMAN
Immunogen Region: 1-145
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
Immunogen Sequence: MAVLAALLRSGARSRSPLLR RLVQEIRYVERSYVSKPTLK EVVIVSATRTPIGSFLGSLS LLPATKLGSIAIQGAIEKAG IPKEEVKEAYMGNVLQGGEG QAPTRQAVLGAGLPISTPCT TINKVCASGMKAIMMASQSL MCGHQ
Post Translational Modifications Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5.
Function This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
Protein Name Acetyl-Coa Acetyltransferase - Mitochondrial
Acetoacetyl-Coa Thiolase
T2
Database Links Reactome: R-HSA-70895
Reactome: R-HSA-77108
Reactome: R-HSA-77111
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Acetyl-Coa Acetyltransferase - Mitochondrial antibody
Anti-Acetoacetyl-Coa Thiolase antibody
Anti-T2 antibody
Anti-ACAT1 antibody
Anti-ACAT antibody
Anti-MAT antibody

Information sourced from Uniprot.org

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