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Anti-ACAN antibody (420-656) (STJ113946)

Western blot analysis of extracts of mouse brain, using Aggrecan antibody (STJ113946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of mouse cartilage cells using Aggrecan rabbit polyclonal antibody (STJ113946) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat cartilage cells using Aggrecan antibody (STJ113946) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACAN antibody (420-656) (STJ113946)

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STJ113946

£112.50 - £522.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IF
Reactivity:	Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-ACAN (420-656) is suitable for use in Western Blot and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	ACAN
Gene ID:	176
Uniprot ID:	PGCA_HUMAN
Immunogen Region:	420-656
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 420-656 of human Aggrecan (NP_037359.3).
Immunogen Sequence:	ATRPWGFPTPGLGPATAFTS EDLVVQVTAVPGQPHLPGGV VFHYRPGPTRYSLTFEEAQQ ACLRTGAVIASPEQLQAAYE AGYEQCDAGWLRDQTVRYPI VSPRTPCVGDKDSSPGVRTY GVRPSTETYDVYCFVDRLEG EVFFATRLEQFTFQEA

Tissue Specificity	Detected in fibroblasts (at protein level). Restricted to cartilage.
Post Translational Modifications	Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.
Function	This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Protein Name	Aggrecan Core Protein Cartilage-Specific Proteoglycan Core Protein Cspcp Chondroitin Sulfate Proteoglycan Core Protein 1 Chondroitin Sulfate Proteoglycan 1 Cleaved Into - Aggrecan Core Protein 2
Database Links	Reactome: R-HSA-1474228 Reactome: R-HSA-2022854 Reactome: R-HSA-2022857 Reactome: R-HSA-3000178 Reactome: R-HSA-3656225 Reactome: R-HSA-3656243 Reactome: R-HSA-3656244
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Aggrecan Core Protein antibody Anti-Cartilage-Specific Proteoglycan Core Protein antibody Anti-Cspcp antibody Anti-Chondroitin Sulfate Proteoglycan Core Protein 1 antibody Anti-Chondroitin Sulfate Proteoglycan 1 Cleaved Into - Aggrecan Core Protein 2 antibody Anti-ACAN antibody Anti-AGC1 antibody Anti-CSPG1 antibody Anti-MSK16 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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