• Western blot analysis of extracts of various cell lines, using ABHD5 rabbit monoclonal antibody (STJ11102351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using ABHD5 Rabbit monoclonal antibody (STJ11102351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ABHD5 antibody (100-200) [S1MR] (STJ11102351)

SKU:
STJ11102351

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ABHD5 (100-200) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABHD5
Gene ID: 51099
Uniprot ID: ABHD5_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ABHD5 (Q8WTS1).
Immunogen Sequence: TNRPVYAFDLLGFGRSSRPR FDSDAEEVENQFVESIEEWR CALGLDKMILLGHNLGGFLA AAYSLKYPSRVNHLILVEPW GFPERPDLADQDRPIPVWIR A
Tissue Specificity Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level).
Function Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfer of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors. Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion.
Protein Name 1-Acylglycerol-3-Phosphate O-Acyltransferase Abhd5
Abhydrolase Domain-Containing Protein 5
Lipid Droplet-Binding Protein Cgi-58
Database Links Reactome: R-HSA-163560
Cellular Localisation Cytoplasm
Lipid Droplet
Cytosol
Colocalized With Plin And Adrp On The Surface Of Lipid Droplets
The Localization Is Dependent Upon The Metabolic Status Of The Adipocytes And The Activity Of Pka
Alternative Antibody Names Anti-1-Acylglycerol-3-Phosphate O-Acyltransferase Abhd5 antibody
Anti-Abhydrolase Domain-Containing Protein 5 antibody
Anti-Lipid Droplet-Binding Protein Cgi-58 antibody
Anti-ABHD5 antibody
Anti-NCIE2 antibody
Anti-CGI-58 antibody

Information sourced from Uniprot.org

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