• Immunofluorescence analysis of HeLa cells using ABCG8 Rabbit polyclonal antibody (STJ22469) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ABCG8 Rabbit polyclonal antibody (STJ22469) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 180s.

Anti-ABCG8 antibody (1-320) (STJ22469)

SKU:
STJ22469

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCG8 (1-320) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCG8
Gene ID: 64241
Uniprot ID: ABCG8_HUMAN
Immunogen Region: 1-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ABCG8 (NP_071882.1).
Immunogen Sequence: MAGKAAEERGLPKGATPQDT SGLQDRLFSSESDNSLYFTY SGQPNTLEVRDLNYQVDLAS QVPWFEQLAQFKMPWTSPSC QNSCELGIQNLSFKVRSGQM LAIIGSSGCGRASLLDVITG RGHGGKIKSGQIWINGQPSS PQLVRKCVAHVRQHNQLLPN LTVRETLAFIAQMRLPRTFS QAQRDKRVEDVIAELRLRQC ADTRVGNMYVRGLSGGERRR VSIGVQLLWNPGILILDEP
Tissue Specificity Predominantly expressed in the liver. Low expression levels in the small intestine and colon. Very low levels in other tissues, including brain, heart and spleen.
Post Translational Modifications N-glycosylated.
Function ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.
Protein Name Atp-Binding Cassette Sub-Family G Member 8
Sterolin-2
Database Links Reactome: R-HSA-1369062
Reactome: R-HSA-5679090
Reactome: R-HSA-5679096
Reactome: R-HSA-9029569
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family G Member 8 antibody
Anti-Sterolin-2 antibody
Anti-ABCG8 antibody

Information sourced from Uniprot.org

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