| Host: | Goat |
| Applications: | Pep-ELISA/WB/FC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ABCB5 (Internal) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80kDa band observed in lysates of cell line A431 (calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.F |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ABCB5 |
| Gene ID: | 340273 |
| Uniprot ID: | ABCB5_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_848654.3; NP_001157413.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1) |
| Immunogen Sequence: | QTQHRNTSKKAQ |
| Function | Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair. |
| Protein Name | Atp-Binding Cassette Sub-Family B Member 5Abcb5 P-GpP-Glycoprotein Abcb5 |
| Database Links | Reactome: R-HSA-382556 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Atp-Binding Cassette Sub-Family B Member 5 antibodyAnti-Abcb5 P-Gp antibodyAnti-P-Glycoprotein Abcb5 antibodyAnti-ABCB5 antibody |
Information sourced from Uniprot.org