• Western blot analysis of extracts of Rat brain, using ABCA1 Polyclonal Antibody (STJ118765) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Western blot analysis of extracts of various cell lines, using ABCA1 Polyclonal Antibody (STJ118765) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded mouse kidney using ABCA1 rabbit polyclonal antibody (STJ118765) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat ovary using ABCA1 rabbit polyclonal antibody (STJ118765) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using ABCA1 rabbit polyclonal antibody (STJ118765) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ABCA1 antibody (1870-2120) (STJ118765)

SKU:
STJ118765

£166.50 - £446.50
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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-ABCA1 (1870-2120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: ABCA1
Gene ID: 19
Uniprot ID: ABCA1_HUMAN
Immunogen Region: 1870-2120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1870-2120 of human ABCA1 (NP_005493.2).
Tissue Specificity Widely expressed, but most abundant in macrophages.
Post Translational Modifications Phosphorylation on Ser-2054 regulates phospholipid efflux. Palmitoylated by ZDHHC8. Palmitoylation is essential for localization to the plasma membrane.
Function Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates in phospholipid transfer to apoliproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apoliproteins and the formation of nascent high density lipoproteins/HDLs.
Protein Name Phospholipid-Transporting Atpase Abca1
Atp-Binding Cassette Sub-Family A Member 1
Atp-Binding Cassette Transporter 1
Abc-1
Atp-Binding Cassette 1
Cholesterol Efflux Regulatory Protein
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-5682113
Reactome: R-HSA-8963896
Reactome: R-HSA-9029569
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Endosome
Alternative Antibody Names Anti-Phospholipid-Transporting Atpase Abca1 antibody
Anti-Atp-Binding Cassette Sub-Family A Member 1 antibody
Anti-Atp-Binding Cassette Transporter 1 antibody
Anti-Abc-1 antibody
Anti-Atp-Binding Cassette 1 antibody
Anti-Cholesterol Efflux Regulatory Protein antibody
Anti-ABCA1 antibody
Anti-ABC1 antibody
Anti-CERP antibody

Information sourced from Uniprot.org

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