• Western blot analysis of lysates from COS7 cells, using AAK1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using AAK1 Polyclonal Antibody

Anti-AAK1 antibody (240-320 Internal) (STJ91403)

SKU:
STJ91403

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ICC, ELISA
Reactivity: Human, Mouse, Rat, Monkey
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Ap2-Associated Protein Kinase 1 (240-320 Internal) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AAK1
Gene ID: 22848
Uniprot ID: AAK1_HUMAN
Immunogen Region: 240-320 Internal
Specificity: AAK1 polyclonal antibody (Ap2-Associated Protein Kinase 1) binds to endogenous Ap2-Associated Protein Kinase 1 at the amino acid region 240-320 Internal.
Immunogen: The antiserum was produced against synthesized peptide derived from human AAK1 at amino acid range 271-320
Post Translational Modifications Autophosphorylated.
Function Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue.
Protein Name Ap2-Associated Protein Kinase 1
Adaptor-Associated Kinase 1
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Membrane
Clathrin-Coated Pit
Cell Junction
Synapse
Presynapse
Active When Found In Clathrin-Coated Pits At The Plasma Membrane
In Neuronal Cells
Enriched At Presynaptic Terminals
In Non-Neuronal Cells
Enriched At Leading Edge Of Migrating Cells
Alternative Antibody Names Anti-Ap2-Associated Protein Kinase 1 antibody
Anti-Adaptor-Associated Kinase 1 antibody
Anti-AAK1 antibody
Anti-KIAA1048 antibody

Information sourced from Uniprot.org

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