• Western blot analysis of extracts from Hep G2 cells, using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of Alpha-2-Macroglobulin (A2M) in paraffin-embedded human cervix cancer tissue using Alpha-2-Macroglobulin (A2M) rabbit monoclonal antibody (STJ11102540) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Alpha-2-Macroglobulin (A2M) in paraffin-embedded human tonsil tissue using Alpha-2-Macroglobulin (A2M) rabbit monoclonal antibody (STJ11102540) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Alpha-2-Macroglobulin (A2M) in paraffin-embedded mouse brain tissue using Alpha-2-Macroglobulin (A2M) rabbit monoclonal antibody (STJ11102540) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Alpha-2-Macroglobulin (A2M) in paraffin-embedded rat spleen tissue using Alpha-2-Macroglobulin (A2M) rabbit monoclonal antibody (STJ11102540) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of rat liver using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human liver using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse liver using Alpha-2-Macroglobulin (Alpha-2-Macroglobulin (A2M) ) rabbit monoclonal antibody (STJ11102540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-A2M antibody (1200-1300) [S0MR] (STJ11102540)

SKU:
STJ11102540

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:200-1:2000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: A2M
Gene ID: 2
Uniprot ID: A2MG_HUMAN
Immunogen Region: 1200-1300
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1200-1300 of human Alpha-2-Macroglobulin (A2M) (P01023).
Immunogen Sequence: QAPSAEVEMTSYVLLAYLTA QPAPTSEDLTSATNIVKWIT KQQNAQGGFSSTQDTVVALH ALSKYGAATFTRTGKAAQVT IQSSGTFSSKFQVDNNNRLL L
Tissue Specificity Secreted in plasma.
Function Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Protein Name Alpha-2-Macroglobulin
Alpha-2-M
C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140837
Reactome: R-HSA-1474228
Reactome: R-HSA-8963896
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-2-Macroglobulin antibody
Anti-Alpha-2-M antibody
Anti-C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5 antibody
Anti-A2M antibody
Anti-CPAMD5 antibody
Anti-FWP007 antibody

Information sourced from Uniprot.org

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