• Confocal imaging of paraffin-embedded Mouse brain using 5HT7 Receptor Rabbit monoclonal antibody (STJ11103088, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat brain using 5HT7 Receptor Rabbit monoclonal antibody (STJ11103088, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using 5HT7 Receptor Rabbit monoclonal antibody (STJ11103088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-5HT7 Receptor antibody (1-100) [S8MR] (STJ11103088)

SKU:
STJ11103088

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:2000
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HTR7
Gene ID: 3363
Uniprot ID: 5HT7R_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 5HT7 Receptor (P34969).
Immunogen Sequence: MMDVNSSGRPDLYGHLRSFL LPEVGRGLPDLSPDGGADPV AGSWAPHLLSEVTASPAPTW DAPPDNASGCGEQINYGRVE KVVIGSILTLITLLTIAGNC
Tissue Specificity Isoform A: Predominant isoform in spleen, caudate and hippocampus. Isoform B: Expressed at lower levels. Isoform D: Minor isoform in terms of expression.
Function G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR7 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.
Protein Name 5-Hydroxytryptamine Receptor 7
5-Ht-7
5-Ht7
5-Ht-X
Serotonin Receptor 7
Database Links Reactome: R-HSA-390666
Reactome: R-HSA-418555
Reactome: R-HSA-9706019 P34969-2
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-5-Hydroxytryptamine Receptor 7 antibody
Anti-5-Ht-7 antibody
Anti-5-Ht7 antibody
Anti-5-Ht-X antibody
Anti-Serotonin Receptor 7 antibody
Anti-HTR7 antibody

Information sourced from Uniprot.org

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