• Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of lysates from HT29 cells and RAW264.7 cells, using 14-3-3 sigma Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-14-3-3 sigma antibody (STJ91377)

SKU:
STJ91377

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal anti-14-3-3 sigma antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: IHC-p 100-300
WB 1:500-1:2000
ELISA 1:20000
Specificity: 14-3-3 sigma polyclonal antibody detects endogenous levels of 14-3-3 sigma protein.
Uniprot ID: 1433S_HUMAN
Gene ID: 2810
Gene Symbol: SFN
Immunogen: Synthesized peptide derived from human 14-3-3 sigma
Immunogen Region: 10-90 aa, Internal
Post Translational Modifications Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. p53-regulated inhibitor of G2/M progression.
Protein Name 14-3-3 Protein Sigma
Epithelial Cell Marker Protein 1
Stratifin
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-6804114
Reactome: R-HSA-75035
Reactome: R-HSA-9614399
Cellular Localisation Cytoplasm
Nucleus
Secreted
May Be Secreted By A Non-Classical Secretory Pathway
Alternative Antibody Names Anti-14-3-3 Protein Sigma antibody
Anti-Epithelial Cell Marker Protein 1 antibody
Anti-Stratifin antibody
Anti-SFN antibody
Anti-HME1 antibody

Information sourced from Uniprot.org

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