• Immunofluorescence analysis of HeLa cells, using 14-3-3 eta Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from Jurkat cells, using 14-3-3 eta Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-14-3-3 eta antibody (STJ91374)

SKU:
STJ91374

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, IF, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-14-3-3 eta antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Specificity: 14-3-3 eta polyclonal antibody detects endogenous levels of 14-3-3 eta protein.
Uniprot ID: 1433F_HUMAN
Gene ID: 7533
Gene Symbol: YWHAH
Immunogen: Synthesized peptide derived from human 14-3-3 eta
Immunogen Region: 30-110 aa, Internal
Post Translational Modifications Phosphorylated on Ser-59 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Protein Name 14-3-3 Protein Eta
Protein As1
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-1445148
Reactome: R-HSA-5625740
Reactome: R-HSA-5628897
Reactome: R-HSA-75035
Alternative Antibody Names Anti-14-3-3 Protein Eta antibody
Anti-Protein As1 antibody
Anti-YWHAH antibody
Anti-YWHA1 antibody

Information sourced from Uniprot.org

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