Species

Secondary Antibodies by Species

Species‑Specific Secondary Antibodies

Matched to your primary antibody's host species for clean, specific detection

Choosing a secondary antibody that is raised against the host species of your primary antibody is essential for reducing background and avoiding cross‑reactivity. Our range includes secondary antibodies raised in goat, donkey, rabbit, chicken and mouse, with reactivity against immunoglobulins from human, mouse, rat, rabbit, goat, chicken and many other species.

Browse by species

Find secondary antibodies by target species

Select the species of your primary antibody to view matching secondary antibodies.

Anti‑Cat Anti‑Chicken Anti‑Cow Anti‑Donkey Anti‑Goat Anti‑Guinea Pig Anti‑Horse
Anti‑Human Anti‑Monkey Anti‑Mouse Anti‑Pig Anti‑Rabbit Anti‑Rat Anti‑Sheep
How it works

Matching host and target species for clean detection

A secondary antibody must recognise the host species of your primary antibody. For example, if your primary antibody is raised in mouse, you need an anti‑mouse secondary antibody. Our catalogue is organised by the species of the primary antibody that each secondary antibody will recognise, making it straightforward to find the right match. We also offer cross‑adsorbed options to minimise reactivity with related immunoglobulins from other species, giving you cleaner results in multiplex or tissue‑based assays.

Category benefits

Why choose our species‑specific secondary antibodies?

✓ Broad species reactivity
Our secondary antibodies are available with reactivity against immunoglobulins from human, mouse, rat, rabbit, goat, chicken, donkey, sheep, guinea pig, hamster and many other species, so you can find the right match for your primary antibody.
✓ Multiple host species for the secondary antibody
We offer secondary antibodies raised in goat, donkey, rabbit, chicken and mouse, giving you flexibility to avoid host‑species conflicts in your experimental system.
✓ Cross‑adsorbed for low background
Many of our species‑specific antibodies are highly cross‑adsorbed to remove antibodies that react with immunoglobulins from other species, reducing non‑specific binding in multiplex IHC, ICC and flow cytometry.
✓ Wide conjugate choice
Species‑specific secondaries are available with HRP, biotin, FITC, Alexa Fluor®, DyLight®, Rhodamine, ABflo® and other conjugates, so you can match your detection system without changing your primary antibody.
Where they're used

Typical applications

✓ Multiplex immunohistochemistry
Cross‑adsorbed secondary antibodies raised in different host species allow you to detect multiple primary antibodies from different species in the same tissue section without cross‑reactivity.
✓ Flow cytometry
Species‑specific fluorophore‑conjugated secondaries are used for cell surface and intracellular staining, enabling clear discrimination of cell populations in mixed‑species samples.
✓ Western blot
HRP‑conjugated secondary antibodies matched to the primary antibody's species are used with chemiluminescent substrates for sensitive protein detection on membranes.
✓ ELISA
Enzyme‑ or biotin‑conjugated species‑specific secondaries are used as detection reagents in sandwich and indirect ELISA formats, providing signal amplification with minimal background.

Unsure which species‑specific secondary antibody to choose?

Contact our technical support team for help matching the host species and isotype of your primary antibody. We can also advise on cross‑adsorbed options for multiplex experiments. Each product page includes a downloadable datasheet with validated dilution ranges and application recommendations.

Looking for secondary antibodies by species?

Browse our full range of species‑specific secondary antibodies, with reactivity against human, mouse, rat, rabbit, goat, chicken and many other species, available in a wide range of conjugates.

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