Conjugated Secondary Antibodies | St John's Laboratory

To use the Alexa Fluors with fluorescent imagers, use a spectral line of the blue laser diode for Alexa Fluors 405, a cyan (488 nm) laser for Alexa Fluors 488, a yellow (526 nm) laser for Alexa Fluor 550 or 594, and a red (633 nm) laser for Alexa Fluor 649. The Alexa Fluor 680 and 790 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, and 800 nm

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 750} (STJS000691)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£155.00 - £951.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Alexa Fluor 790} (STJS000734)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/IF/FC

Reactivity :

Rabbit

£155.00 - £951.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Biotin} (STJ99670)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rabbit

£79.00 - £193.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Biotin} (STJS000152)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Rabbit

£72.00 - £229.00

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Zhang, Zi-Bin, et al. "miRNA-7a-2-3p Inhibits Neuronal Apoptosis in Oxygen-Glucose Deprivation (OGD) Model. " Frontiers in neuroscience 13 (2019).

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {FITC} (STJ99668)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rabbit

£79.00 - £193.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {FITC} (STJS000184)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/FC

Reactivity :

Rabbit

£72.00 - £229.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {Gold} (STJ99356)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Rabbit

£125.00

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TBBPA causes apoptosis in grass carp hepatocytes involving destroyed ER-mitochondrial function. CHEMOSPHERE Shiwen Xu WB Fish 1:8000 L8824 cell

Goat Anti-Rabbit IgG Heavy & Light Chain antibody {HRP} (STJ99666)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/ELISA

Reactivity :

Rabbit

£79.00 - £193.00

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Goat Anti-Rabbit IgG Heavy & Light Chain antibody {HRP} (STJS000120)

Clonality :

Polyclonal

Host :

Goat

Applications :

ELISA/WB/IHC/ICC

Reactivity :

Rabbit

£72.00 - £229.00

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 488} (STJS000813)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 555} (STJS000804)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 568} (STJS000822)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 594} (STJS000832)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 633} (STJS000840)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H&L) antibody {Alexa Fluor 647} (STJS000850)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rabbit

£210.50

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Confocal imaging of paraffin-embedded Mouse brain using Beta III-Tubulin Rabbit mAb (STJ1111133, dilution 1:200) followed by a further incubation with FITC F (ab NA ) ₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific (STJS001213, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Goat Anti-Rabbit IgG, Fc fragment specific Fc antibody {FITC} (STJS001213)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rabbit

£68.50 - £114.50

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Goat Anti-Rat IgG (Full length) antibody {DyLight®405} (STJ140234)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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Immunostaining- C57BL/6J mouse retina labeled with: Green- 1st antibody Cone Arrestin (1:1,000) ; 2nd antibody goat anti-rat (STJ140235, 1:1,000) , Red- 1st antibody Rhodopsin (1:1,000) ; 2nd antibody goat anti-mouse (STJ140232, 1:1,000) , Blue- Nuclear staining (DAPI) , Scale bar = 40 um

Goat Anti-Rat IgG (Full length) antibody {DyLight®488} (STJ140235)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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Immunostaining- rd16 (retinal degeneration) mouse retina labeled with: Red- 1st antibody GFAP (1/500) ; 2nd antibody goat anti-rat (STJ140236, 1:1000) , Blue- Nuclear staining (DAPI) , scale bar = 40 um

Goat Anti-Rat IgG (Full length) antibody {DyLight®550} (STJ140236)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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Goat Anti-Rat IgG (Full length) antibody {DyLight®633} (STJ140237)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Rat

£88.50 - £266.50

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Goat Anti-Rat IgG (H&L) antibody {Alexa Fluor 488} (STJS000814)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rat

£210.50

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Goat Anti-Rat IgG (H&L) antibody {Alexa Fluor 555} (STJS000805)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rat

£210.50

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Goat Anti-Rat IgG (H&L) antibody {Alexa Fluor 568} (STJS000823)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rat

£210.50

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Goat Anti-Rat IgG (H&L) antibody {Alexa Fluor 594} (STJS000833)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rat

£210.50

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Goat Anti-Rat IgG (H&L) antibody {Alexa Fluor 633} (STJS000841)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rat

£210.50

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Goat Anti-Rat IgG (H&L) antibody {Alexa Fluor 647} (STJS000851)

Clonality :

Polyclonal

Host :

Goat

Reactivity :

Rat

£210.50

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To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 649. The DyLight 680 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm and 800 nm

Goat Anti-Rat IgG antibody {Dylight 488} (STJS000775)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/FC

Reactivity :

Rat

£94.00 - £345.00

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Goat Anti-Rat IgG antibody {Dylight 549} (STJS000779)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/FC

Reactivity :

Rat

£94.00 - £345.00

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Goat Anti-Rat IgG antibody {Dylight 594} (STJS000783)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/FC

Reactivity :

Rat

£94.00 - £345.00

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Goat Anti-Rat IgG antibody {Dylight 649} (STJS000787)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/FC

Reactivity :

Rat

£94.00 - £345.00

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Western blot analysis of extracts of Rat IgG, using HRP Goat Anti-Rat IgG (H+L) antibody (STJS000857) at 1:1000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Goat Anti-Rat IgG H&L antibody {HRP} (STJS000857)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-P

Reactivity :

Rat

£45.50 - £69.50

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Flow cytometry: 1X10^6 RK13 cells (negative control, left) and RK13-CD20 transfection cells (right) were surface-stained with rat anti-mouse CD20 Antibody (1:100, orange line) or secondary antibody only (blue line). Non-fluorescently stained RK13 and RK13 transfection cells were used as blank control (red line). FITC Goat Anti-Rat IgG (H+L) (STJS001172, 1:100) was used as a secondary antibody.

Goat Anti-Rat IgG H+L antibody {FITC} (STJS001172)

Clonality :

Polyclonal

Host :

Goat

Applications :

IF/ICC/FC

Reactivity :

Rat

£51.50 - £80.50

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Goat Anti-Tree Shrew IgG Heavy & Light Chain antibody {Biotin} (STJ99438)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Tree Shrew

£144.00

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Goat Anti-Tree Shrew IgG Heavy & Light Chain antibody {FITC} (STJ99488)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Tree Shrew

£144.00

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Goat Anti-Tree Shrew IgG Heavy & Light Chain antibody {Gold} (STJ99388)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Tree Shrew

£144.00

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Goat Anti-Tree Shrew IgG Heavy & Light Chain antibody {HRP} (STJ99538)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC/IF/ELISA

Reactivity :

Tree Shrew

£144.00

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Mouse Anti-Chicken IgY antibody {Biotin} (STJ99433)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Chicken

£240.00

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Mouse Anti-Chicken IgY antibody {Gold} (STJ99383)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Chicken

£240.00

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Mouse Anti-Chicken IgY antibody {HRP} (STJ99533)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Chicken

£240.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 350} (STJS000238)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 405} (STJS000281)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 488} (STJS000324)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 532} (STJS000367)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 555} (STJS000410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 568} (STJS000453)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 594} (STJS000496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 633} (STJS000539)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Mouse Anti-Chicken IgY Heavy & Light Chain antibody {Alexa Fluor 647} (STJS000582)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/FC

Reactivity :

Chicken

£240.00 - £1,075.00

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Conjugation