Signal Transduction | Research Area | St John's Laboratory

Anti-Phospho-CDK4-Thr172 antibody (STJA0009886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western Blot analysis of mouse lung tissue, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CDK5-Ser159 antibody (STJA0009913)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of HT29+PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody

Anti-Phospho-CDK5-Tyr15 antibody (1-50 aa) (STJ90540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK9-T186 Rabbit pAb (STJ117908) at 1:1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK9-T186 antibody (STJ117908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of 3T3 cells using Phospho-Cdk9 (T186) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-CDK9-Thr186 antibody (152-201 aa) (STJ90525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CDKN1A-Ser129 antibody (STJA0010060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-p21 (T145) Polyclonal Antibody

Anti-Phospho-CDKN1A-Thr145 antibody (111-160 aa) (STJ90360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-CDKN1A-Thr57 antibody (STJA0010067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-p27 (S10) Polyclonal Antibody

Anti-Phospho-CDKN1B-Ser10 antibody (1-50 aa) (STJ90361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CDKN1B-Ser178 antibody (144-193 aa) (STJ91151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from HeLa cells, using Phospho-CDKN1B/p27KIP1-T187 Rabbit pAb (STJ22066). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CDKN1B-T187 antibody (STJ22066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€158.13 - €773.13

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Anti-Phospho-CDKN1B-Thr157 antibody (123-172 aa) (STJ91150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-p27 (T187) Polyclonal Antibody

Anti-Phospho-CDKN1B-Thr187 antibody (149-198 aa) (STJ90443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of VEC cells using Phospho-p27 (T198) Polyclonal Antibody

Anti-Phospho-CDKN1B-Thr198 antibody (149-198 aa) (STJ90519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells, using Centromeric Protein A (Phospho-Ser7) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CENPA-Ser7 antibody (1-50 aa) (STJ91332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CFL1-S3 Rabbit pAb (STJ26176). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CFL1-S3 antibody (STJ22068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-Phospho-CFL1-Ser-3 antibody (STJA0005346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€456.88

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Western blot analysis of NIH-3T3 cells using Phospho-Cofilin (S3) Polyclonal Antibody

Anti-Phospho-CFL1-Ser3 antibody (1-50 aa) (STJ90230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (STJ11105777) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-Phospho-CFL1-Y139 antibody (STJ11105777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CFTR-Ser737 antibody (STJ193221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-Che-1/AATF-Ser477 antibody (STJA0003595)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Canine/Mouse/Non-Human Primates/Rat/Sheep/Xenopus

€610.63

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Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit monoclonal antibody (STJ11104149) at1:2000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-CHEK1 antibody [S4149RM] (STJ11104149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€148.13 - €651.88

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S280 Rabbit pAb (STJ22071) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CHEK1-S280 antibody (STJ22071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of 293T, using Phospho-Chk1 (S280) Rabbit monoclonal antibody (STJ11105881) at 1:500 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-CHEK1-S280 antibody [S5881RM] (STJ11105881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Western blot analysis of 293T, using Phospho-Chk1-S280 Rabbit monoclonal antibody (STJ11105894) at 1:2000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-Phospho-CHEK1-S280 antibody [S5894RM] (STJ11105894)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Western blot analysis of lysates from 293T cells, using Phospho-Chk1-S296 Rabbit mAb (STJ11102618) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0. 5 uM) at 37 °C for 24 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-CHEK1-S296 antibody [S8MR] (STJ11102618)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Western blot analysis of lysates from MCF7 cells, using Phospho-Chk1-S317 Rabbit pAb (STJ22072). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CHEK1-S317 antibody (STJ22072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Chk1-S345 Rabbit pAb (STJ29344) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-CHEK1-S345 antibody (STJ29344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of 293T cells using Phospho-Chk1 (S280) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser280 antibody (251-300 aa) (STJ90221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser286 antibody (256-305 aa) (STJ90705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from HUVEC cells treated with UV 15', using Chk1 (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser296 antibody (266-315 aa) (STJ90849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of 293T cells using Phospho-Chk1 (S301) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CHEK1-Ser301 antibody (271-320 aa) (STJ90706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of 293T cells using Phospho-Chk1 (S317) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser317 antibody (286-335 aa) (STJ90222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser345 antibody (310-359 aa) (STJ90223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-Chimaerin beta 2 antibody (STJ502193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€621.88

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Anti-Phospho-Chimaerin beta 2 antibody {Biotin} (STJ502194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€689.38

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Anti-Phospho-Chimaerin beta 2 antibody {FITC} (STJ502195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

€689.38

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Anti-Phospho-Chk1-Ser280 antibody [R32-4D-1] (STJA0035702)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€166.88 - €351.88

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Anti-Phospho-Chk1-Ser296 antibody [R02-4Z6] (STJA0036032)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€166.88 - €351.88

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Anti-Phospho-Chk1-Ser317 antibody [PT0744R] (STJA0040059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-Chk1-Ser345 antibody [PT0651R] (STJA0039191)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€356.25 - €675.00

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Western blot analysis of lysates from HeLa cells, using Phospho-IKK Alpha-S180 Rabbit pAb (STJ110846) at 1:1000 dilution. HeLa cells were treated by both TNF-Alpha (20ng/mL) and Calyculin A (100nM) for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CHUK-S180 antibody (STJ110846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€158.13 - €773.13

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Western blot analysis of various cells using Phospho-IKK Alpha (T23) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CHUK-Thr23 antibody (15-64 aa) (STJ90300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from 293T cells, using Phospho-c-Jun-S63 Rabbit pAb (STJ11103730) at 1:1000 dilution. 293T cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-cJun-S63 antibody (STJ11103730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (STJ11103749) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T91 antibody (STJ11103749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (STJ11103751) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T93 antibody (STJ11103751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€158.13 - €773.13

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Anti-Phospho-Cofilin 1-Ser-3 antibody (STJA0041140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€678.13

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Anti-Phospho-Cofilin antibody (1-50) (STJ506121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€621.88

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