Programmed Cell Death | Research Area | St John's Laboratory

Immunohistochemistry analysis of HMGB1 in paraffin-embedded mouse heart using HMGB1 Rabbit polyclonal antibody (STJ24039) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HMGB1 antibody (69-215) (STJ24039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-HMGB1 antibody (Met1-Glu215) [1A068] (STJA0021906)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$291.87 - $400.89

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Immunofluorescence analysis of Hela cell. 1, HMG-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HMGB1 antibody (N-Term) (STJ96867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-HMGB1 antibody [PT0499R] (STJA0039474)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-HMGB1 antibody [SAA0503] (STJA0019346)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

$289.11 - $1,840.23

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g HMGB2 Rabbit mAb (STJ11102444) , along with a Goat Anti-Rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of HMGB2 in representative gene loci (PTBP1) , as shown in figure.

Anti-HMGB2 antibody (1-100) [S4MR] (STJ11102444)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

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Western blot analysis of various lysates using HMGB2 Rabbit polyclonal antibody (STJ119486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMGB2 antibody (100-200) (STJ119486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various cells using HMG-2 Polyclonal Antibody

Anti-HMGB2 antibody (131-180 aa) (STJ93552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of extracts of RAW264. 7 cells, using HMGB2 antibody (STJ24041). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-HMGB2 antibody (200-300) (STJ24041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-Hsp90 Alpha antibody [SRM14914] (STJ11109644)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Hsp90 Alpha antibody (STJ11102219). Western blot was performed from the immunoprecipitate using Hsp90 Alpha antibody (STJ11102219) at a dilution of 1:1000.

Anti-HSP90AA1 antibody (1-100) [S9MR] (STJ11102219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of various lysates, using HSP90AA1 Rabbit polyclonal antibody (STJ11105239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AA1 antibody (433-732 aa) (STJ11105239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunofluorescence analysis of U2OS cells using Hsp90 Alpha Rabbit polyclonal antibody (STJ24087). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-HSP90AA1 antibody (433-732) (STJ24087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of HSP90 in K562, C6, Hela lysates using Hsp90 alpha antibody.

Anti-HSP90AA1 antibody (62-75 aa) [R06-9C1] (STJA0010746)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$365.01

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Western blot analysis of various lysates using Hsp90 Alpha Rabbit pAb (STJ114322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HSP90AA1 antibody (629-732) (STJ114322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSP90 a Mouse mAb diluted at 1:2, 000.

Anti-HSP90AA1 antibody (660-740 aa) [1F6] (STJ97496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP90AA1 antibody (683-732 aa) (STJ93622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot detection of Hsp90 in Hela, 3T3, C6, K562 and A549 cell lysates using Hsp90 mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AA1 antibody [4B6-G2-H6] (STJ99220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

$205.62 - $496.80

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Western blot detection of Hsp90 alpha in R Brain, Jurkat, CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Hsp90 alpha mouse mAb (1:1000 diluted). Predicted band size:90KDa. Observed band size:90KDa.

Anti-HSP90AA1 antibody [6H7-A3-2G6] (STJ99288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

$205.62 - $496.80

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Western blot analysis of lysates from Recombinant Human IL-1 alpha Protein (STJP000874) using IL-1 alpha Rabbit mAb (STJ11107358) at 1:2000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 10ng, 5ng Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-IL-1 alpha antibody (113-271 aa) [SRM5445] (STJ11107358)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Anti-IL-1 antibody [CRM56] {FITC} (STJA0042687)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$222.87 - $563.73

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Anti-IL-1 antibody [CRM56] {PE} (STJA0042688)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

$222.87 - $563.73

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Anti-IL-1 beta antibody [SM0462] (STJA0010462)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

$235.29 - $322.23

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Anti-IL-1 Beta antibody {ABflo® 488} (STJ11108624)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$0.00

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Anti-IL-1 Beta antibody {ABflo® 594} (STJ11108625)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC

Reactivity :

Human

$0.00

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Anti-IL1 Beta antibody [SRM13313] (STJ11108043)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Human

$0.00

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Anti-IL1 Beta antibody [SRM14700] (STJ11109430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$0.00

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Anti-IL1 Beta antibody [SRM14856] (STJ11109586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$0.00

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Anti-IL18 antibody (1-193 aa) [SRM5148] (STJ11106987)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human/Mouse/Rat

$174.57 - $765.21

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Anti-IL18 antibody (150-200) (STJ506215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$589.95

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Anti-IL18 antibody (150-200) {Biotin} (STJ506216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$729.33

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Anti-IL18 antibody (150-200) {FITC} (STJ506217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$729.33

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Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IL18 antibody (STJ24170). Western blot was performed from the immunoprecipitate using IL18 antibody (STJ24170) at a dilution of 1:1000.

Anti-IL18 antibody (37-193) (STJ24170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$152.49 - $697.59

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Anti-IL18 antibody (37-193) (STJ506218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$589.95

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Western blot analysis of various lysates, using IL18 antibody (STJ11104610) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-IL18 antibody (37-193) [S4610RM] (STJ11104610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $765.21

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Anti-IL18 antibody (37-193) {Biotin} (STJ506219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$729.33

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Anti-IL18 antibody (37-193) {FITC} (STJ506220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$729.33

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IL18 antibody (STJ192030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-IL18 antibody [PT0148R] (STJA0039712)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$207.00 - $745.20

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Anti-IL18 antibody [SRM13359] (STJ11108089)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$0.00

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Anti-IL18 antibody [SRM14586] (STJ11109316)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-IL18/IL1F4 antibody (Tyr37-Asp193) [1A151] (STJA0021976)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$291.87 - $400.89

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Anti-IL18/IL1F4 antibody [Fs0368] (STJA0032126)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Block/ELISA/FCM

Reactivity :

Human

$507.15

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Anti-IL18/IL1F4 antibody [SAA0388] (STJA0027567)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $414.69

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Anti-IL18/IL1F4 antibody [SAA0388] {APC} (STJA0029561)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $505.77

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Anti-IL18/IL1F4 antibody [SAA0388] {FITC} (STJA0028595)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

$319.47 - $411.93

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Anti-IL18/IL1F4 antibody [SAA0388] {PerCP} (STJA0030527)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

$366.39 - $475.41

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Anti-IL18/IL1F4 antibody [SAA0388] {PE} (STJA0031493)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC

Reactivity :

Human

$389.85 - $505.77

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