Muscle Contraction | Research Area | St John's Laboratory

Western blot analysis of lysates from 293T cells, using Phospho-PKA C-alpha (PRKACA) -T197 Rabbit pAb (STJ113495) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKACA-T197 antibody (STJ113495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-proANP antibody (STJ502098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-proANP antibody {FITC} (STJ502100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-PXN-Ser-178 antibody (STJA0005517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£367.50

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Anti-Phospho-PXN-Ser178 antibody (STJ90613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-PXN-Ser272 antibody (STJ90604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-PXN-Thr-538 antibody (STJA0005518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-PXN-Tyr 118 antibody (STJA0005516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-PXN-Tyr-31 antibody [M102] (STJA0005514)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of various cells using Phospho-Paxillin (Y118) Polyclonal Antibody

Anti-Phospho-PXN-Tyr118 antibody (85-134 aa) (STJ90379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of various cells using Phospho-Paxillin (Y31) Polyclonal Antibody diluted at 1:1000

Anti-Phospho-PXN-Tyr31 antibody (15-64 aa) (STJ90380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Paxillin (Y88) Polyclonal Antibody

Anti-Phospho-PXN-Tyr88 antibody (STJ90381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using Phospho-Paxillin-Y118 Rabbit pAb (STJ22361) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PXN-Y118 antibody (STJ22361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-Paxillin-Y118 Rabbit mAb (STJ11102648) at 1:1000 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PXN-Y118 antibody [S8MR] (STJ11102648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-RYR2-S2808 antibody (STJ11107697)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-RYR2-S2814 antibody (STJ11107660)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-RYR2-S2830 antibody (STJ11107661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using RyR2 (Phospho-Ser2808) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RYR2-Ser2808 antibody (2774-2823 aa) (STJ91127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Phospho-SERCA2/ATP2A2-Thr484 Rabbit pAb (STJ11107681) at 1:1000 dilution. NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-SERCA2/ATP2A2-Thr484 antibody (STJ11107681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-TLN1-Ser-425 antibody (STJA0005613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-TLN1-Ser425 antibody (STJ196447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human heart using Phospho-Cardiac Troponin I (TNNI3) -S23/S24 Rabbit monoclonal antibody (STJ11105867) at dilution of 1:700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-TNNI3-S23/S24 antibody [S5867RM] (STJ11105867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from Jurkat cells, using TNNI3 (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TNNI3-Ser43 antibody (11-60 aa) (STJ90883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from mouse heart, using TNNI3 (Phospho-Thr142) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TNNI3-Thr142 antibody (111-160 aa) (STJ90481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-VCL-Tyr821 antibody (786-835 aa) (STJ91311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S39 Rabbit pAb (STJ117904) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S39 antibody (STJ117904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (STJ117902) at 1:3000 dilution or Vimentin antibody (STJ249342). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S56 antibody (STJ117902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S83 Rabbit pAb (STJ11101153) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ11101153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 pAb (STJ117897) at 1:2000 dilution or Vimentin antibody (STJ26092). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ117897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Anti-Phospho-VIM-Ser39 antibody (STJ196472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of A549 cells using Phospho-Vimentin (S56) Polyclonal Antibody

Anti-Phospho-VIM-Ser56 antibody (31-80 aa) (STJ90927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody

Anti-Phospho-VIM-Ser83 antibody (56-105 aa) (STJ90926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Phospho-VIM-Tyr38 antibody (STJ98572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysate from Jurkat cells, using phospho-Vimentin (Phospho-Tyr61) antibody.

Anti-Phospho-VIM-Tyr61 antibody (STJ90435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-Vimentin-S34 antibody [YT33] (STJA0019156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Vimentin-S56 antibody [4A4] (STJA0019155)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Vimentin-S83 antibody [MO82] (STJA0019157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-WWTR1-S89 antibody (STJ11101053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-WWTR1-Ser89 antibody (STJ194751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospholamban antibody [SRM14672] (STJ11109402)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/ELISA

Reactivity :

Mouse/Rat

£0.00

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Western blot analysis of various lysates, using PKA C-alpha (PRKACA) antibody (STJ11102940) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PKA C-alpha antibody [S0MR] (STJ11102940)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of HeLa cells, using PLB Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLN antibody (1-50 aa) (STJ95152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PLN antibody (STJ119943) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PLN antibody (1-52) (STJ119943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using PKA C-alpha (PRKACA) Rabbit polyclonal antibody (STJ111027) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKACA antibody (1-351) (STJ111027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using PKAC Alpha Rabbit polyclonal antibody (STJ11105351) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKACA antibody (251-351) (STJ11105351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of cAMP Protein Kinase Catalytic subunit (green) in Hela using cAMP Protein Kinase Catalytic subunit antibody, and DAPI (blue)

Anti-PRKACA antibody (319-332 aa) [R07-8H3] (STJA0011064)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£246.50

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Anti-PRKACA antibody [PT0438R] (STJA0039556)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from wild type (WT) and PKA C-alpha (PRKACA) knockdown (KD) 293T cells, using [KD Validated] PKA C-alpha (PRKACA) Mouse monoclonal antibody (STJ11104590) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRKACA antibody [S4590MM] (STJ11104590)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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