Metabolism Of Proteins | Research Area | St John's Laboratory

Anti-Smad2 antibody [PT0111R] (STJA0039647)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunohistochemistry analysis of Smad3 in paraffin-embedded human tonsil using [KO Validated] Smad3 Rabbit monoclonal antibody (STJ11101652) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SMAD3 antibody (100-200) [S2MR] (STJ11101652)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, Smad3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD3 antibody (145-194 aa) (STJ95699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of NIH/3T3 cells using Smad3 Rabbit polyclonal antibody (STJ11101192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD3 antibody (150-425) (STJ11101192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Smad3 antibody immunocytochemistry A549 cells STJA0010795

Anti-SMAD3 antibody (202-219 aa) [R09-7H4] (STJA0010795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

£133.50 - £281.50

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Immunohistochemical analysis of paraffin-embedded Human Liver Carcinoma Tissue using Smad3 Mouse mAb diluted at 1:200.

Anti-SMAD3 antibody (350-430 aa) [4C9] (STJ97772)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Smad3 antibody western blot 293T Jurkat rat mouse heart STJA0032967

Anti-Smad3 antibody [4H4-10A10-7B4] (STJA0032967)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Anti-Smad3 antibody [PT0830R] (STJA0039185)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Smad3 antibody western blot 3T3 C6 STJA0034867

Anti-Smad3 antibody [R02-1I2] (STJA0034867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Hamster

£133.50 - £281.50

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Anti-Smad3 antibody [SRM13315] (STJ11108045)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IHC-P/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-Smad3 antibody [SRM14491] (STJ11109221)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-DPC4 SMAD4 antibody IHC Human pancreatic ductal adenocarcinoma STJ180086

Anti-SMAD4 antibody (1-552 aa) [B‐8] (STJ180086)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£147.50 - £564.50

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Immunohistochemistry of mouse Colon with Anti-SMAD4 primary antibody (STJ503059) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (131-181) (STJ503059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-SMAD4 antibody (131-181) {Biotin} (STJ503063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (131-181) {FITC} (STJ503064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (136-156) {FITC} (STJ502007)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Immunofluorescence analysis of Hela cell. 1, Smad4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SMAD4 antibody (21-70 aa) (STJ95702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Smad4 antibody immunocytochemistry HeLa cells STJA0012592

Anti-SMAD4 antibody (316-552 aa) [R05-1I7] (STJA0012592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Western blot analysis of lysates from HCT116 cells, using Smad4 Rabbit pAb (STJ27624) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SMAD4 antibody (320-552) (STJ27624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g Smad4 rabbit monoclonal antibody (STJ11101653) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Smad4 in representative gene loci (JUNB) , as shown in figure.

Anti-SMAD4 antibody (320-552) [S3MR] (STJ11101653)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunohistochemistry of mouse liver with Anti-SMAD4 primary antibody (STJ503060) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (400-450) (STJ503060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£400.50

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Immunohistochemistry with Anti-SMAD4 Antibody (STJ503062) at 1:50 dilution in buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 20X magnification. FFPE section.

Anti-SMAD4 antibody (400-450) (STJ503062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Monkey/Mouse/Rat

£400.50

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (400-450) {Biotin} (STJ503069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (400-450) {FITC} (STJ503070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

D.melanogaster/Human/Mouse/Rat

£497.50

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Immunohistochemistry of mouse small intestine with Anti-SMAD4 primary antibody (STJ503061) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SMAD4 antibody (502-552) (STJ503061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-SMAD4 antibody (502-552) {Biotin} (STJ503067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-SMAD4 antibody (502-552) {FITC} (STJ503068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Western blot analysis using Smad4 monoclonal antibody against A431 (1) , SK-N-SH (2) , K562 (3) , HepG2 (4) and HUVE12 (5) cell lysate.

Anti-SMAD4 antibody [4G1C6] (STJ98385)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Smad4 antibody immunohistochemistry paraffin-embedded tonsil tissue STJA0037716

Anti-Smad4 antibody [BP6219] (STJA0037716)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£308.50

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Anti-Smad4 antibody [PT0550R] (STJA0039429)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-DPC4 (SMAD4) antibody IHC human pancreatic ductal carcinoma STJ180565

Anti-SMAD4 antibody [ZR392] (STJ180565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£148.50 - £589.50

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Anti-SMAD7 antibody (N-Term) (STJA0005586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

£319.50

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Western blot WB analysis SMAD7 in HepG2 cells using antibody STJA0041408

Anti-SMAD7 antibody (N-Term) (STJA0041408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

£474.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SMAD7 antibody (STJ192434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Goat/Sheep

£49.00 - £261.00

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Anti-Small ubiquitin-related modifier 1 antibody (aa: 6-21) (STJA0040870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-F/WB

Reactivity :

Human/Rat

£540.50

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Anti-Small ubiquitin-related modifier 1 antibody (aa: 6-21) (STJA0041027)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC-F/WB

Reactivity :

Human/Rat

£540.50

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Western blot analysis of extracts of various cell lines, using SMC1 antibody (STJ29088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMC1A antibody (1-130) (STJ29088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Confocal imaging of HeLa cells using SMC1 Rabbit monoclonal antibody (STJ11102132, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-SMC1A antibody (1134-1233) [S2MR] (STJ11102132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of 293 cells using SMC1 Polyclonal Antibody

Anti-SMC1A antibody (931-980 aa) (STJ95705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot detection of SMC1A (C-term) in MOLT-4, U87 MG and HelaNE cell lysates using SMC1A (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 143KDa.Observed band size: 143KDa.

Anti-SMC1A antibody (C-Term) [4C5-C8-A11] (STJ99054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using SMC1 monoclonal antibody against HeLa nuclear extract.

Anti-SMC1A antibody (N-Term) (STJ98551)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Pig

£149.00 - £360.00

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Western blot detection of SMC1A (N-terminus) in HeLa, Jurkat, and A431 cell lysates using SMC1A (N-terminus) mouse mAb STJ99053

Anti-SMC1A antibody (N-Term) [8E6-A4-F3] (STJ99053)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/FC

Reactivity :

Human

£149.00 - £360.00

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SMC1A antibody western blot MOLT4 U87 MG HeLaNE STJA0032358

Anti-SMC1A antibody [4C5-C8-A11] (STJA0032358)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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SMC1A antibody western blot K562 Jurkat C6 3T3 HeLa STJA0038209

Anti-SMC1A antibody [4C5-C8-A11] (STJA0038209)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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SMC1A antibody western blot K562 rat brain C6 3T3 HeLa STJA0034869

Anti-SMC1A antibody [R05-5D4] (STJA0034869)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-SMC1A antibody [SAA1979] (STJA0020407)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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