Gene Expression And Transcription | Research Area | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, p35 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CDK5R1 antibody (11-60 aa) (STJ94875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various lysates, using CDK5R1 Rabbit monoclonal antibody (STJ11105105) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDK5R1 antibody (200-300 aa) [S5105RM] (STJ11105105)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Immunofluorescence analysis of SH-SY5Y cells using CDK5R1 antibody (STJ116708) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDK5R1 antibody (99-307) (STJ116708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-CDK5R1 antibody [SAA1679] (STJA0020107)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$293.25 - $1,634.61

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Anti-CDK5R1 antibody [SRM13397] (STJ11108127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g CDK6 antibody (STJ11103846). Western blot was performed from the immunoprecipitate using CDK6 antibody (STJ11103846) at a dilution of 1:1000.

Anti-CDK6 antibody (226-326) (STJ11103846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using CDK6 Rabbit monoclonal antibody (STJ11101226) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CDK6 antibody (227-326) [S6MR] (STJ11101226)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-CDK6 antibody (280-325 aa) (STJ98721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-CDK6 antibody [PT0069R] (STJA0039694)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$207.00 - $745.20

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Western blot analysis of lysates from wild type (WT) and CDK7 knockout (KO) 293T cells, using [KO Validated] CDK7 Rabbit polyclonal antibody (STJ114808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK7 antibody (211-346) (STJ114808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of various cells using Cdk7 Polyclonal Antibody

Anti-CDK7 antibody (291-340 aa) (STJ92203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of various lysates using CDK8 Rabbit mAb (STJ11102517) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-CDK8 antibody (1-100) [S7MR] (STJ11102517)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$163.53 - $719.67

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Western blot analysis of various cells using Cdk8 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CDK8 antibody (1-50 aa) (STJ92205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of extracts of various lysates, using CDK8 Rabbit monoclonal antibody (STJ11105214) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDK8 antibody (364-464 aa) [S5214RM] (STJ11105214)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells, using 3 Mu g CDK8 antibody (STJ27494). Western blot was performed from the immunoprecipitate using CDK8 antibody (STJ27494) at a dilution of 1:1000.

Anti-CDK8 antibody (365-464) (STJ27494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of extracts of C6 cells, using CDK9 antibody (STJ11102769) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDK9 antibody (153-372) (STJ11102769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry analysis of paraffin-embedded rat liver using CDK9 Rabbit polyclonal antibody (STJ114907) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDK9 antibody (153-372) (STJ114907)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g CDK9 rabbit monoclonal antibody (STJ11101280) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of CDK9 in representative gene loci (RAC1) , as shown in figure.

Anti-CDK9 antibody (273-372) [S0MR] (STJ11101280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-CDK9 antibody (STJA0010131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis using Cdk9 monoclonal antibody against HEK293 (1) and CDK9-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CDK9 antibody [1B5A7] (STJ97949)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-Cdk9 antibody [PT1054R] (STJA0039967)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Whole cell lysates of A549 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21 (PTR2559) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 21kDaObserved band size: 21kDa

Anti-CDKN1A antibody (1-100 aa) (STJ196154)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse

$67.62 - $360.18

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Immunohistochemistry analysis of CDKN1A/p21CIP1 in paraffin-embedded human breast cancer using CDKN1A/p21CIP1 Rabbit polyclonal antibody (STJ111165) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDKN1A antibody (1-164) (STJ111165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of MCF7, using CDKN1A/p21CIP1 antibody (STJ23069) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDKN1A antibody (1-164) (STJ23069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-CDKN1A antibody (1-164) [S1MR] (STJ11101631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Immunofluorescence analysis of Hela cell. 1, p21 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CDKN1A antibody (111-160 aa) (STJ94862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of p21 in MCF-7 lysates using p21 antibody.

Anti-CDKN1A antibody (148-164 aa) [R07-3K5] (STJA0011727)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$340.17

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Anti-CDKN1A antibody (Full Length) [M513] (STJA0005501)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human

$440.91

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Anti-CDKN1A/p21CIP1 antibody [SRM14465] (STJ11109195)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$0.00

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Anti-CDKN1A/p21CIP1 antibody [SRM15042] (STJ11109772)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/ELISA

Reactivity :

Human/Mouse

$0.00

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Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (STJ119057) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-CDKN1B antibody (1-198) (STJ119057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of U-2 OS cells using p27 KIP 1 antibody (STJ23072) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDKN1B antibody (1-198) (STJ23072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p27Kip1 (ABT-p27) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 22kDaObserved band size: 24kDa

Anti-CDKN1B antibody (100-198 aa) [ABT-p27] (STJ196998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, p27 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CDKN1B antibody (149-198 aa) (STJ94866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Cow

$67.62 - $360.18

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Western blot analysis using p27 monoclonal antibody against CDKN1B-hIgGFc transfected HEK293 cell lysate.

Anti-CDKN1B antibody [3D8] (STJ98298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$205.62 - $496.80

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Human B-cell lymphoma stained with anti-p27 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.

Anti-CDKN1B antibody [SX53G8] (STJ180205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$222.87 - $679.65

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Anti-CDKN1B/p27KIP1 antibody [SRM13057] (STJ11107787)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Western blot analysis of various cells using p16 Polyclonal Antibody

Anti-CDKN2A antibody (10-59 aa) (STJ94858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Whole cell lysates of Hela were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p16 (PTR2301) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 16kDaObserved band size: 16kDa

Anti-CDKN2A antibody (100-156 aa) [PTR2301] (STJ196735)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Confocal imaging of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-CDKN2A antibody (57-156) [S3MR] (STJ113253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

$163.53 - $719.67

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Western blot analysis of various cells using p14 Polyclonal Antibody diluted at 1：500

Anti-CDKN2A antibody (71-120 aa) (STJ94855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of CDKN2A/p14ARF in PC-3 lysates using CDKN2A antibody.

Anti-CDKN2A antibody (75-132 aa) [R01-1Q5] (STJA0015047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human

$365.01

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Western blot analysis of 293T 22RV1 using p16 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-CDKN2A antibody (90-170 aa) (STJ97617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-CDKN2A antibody (94-111) (STJ502022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-CDKN2A antibody (94-111) {Biotin} (STJ502027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-CDKN2A antibody (94-111) {FITC} (STJ502028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-CDKN2A antibody (Mid-Region) (STJ502021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$552.69

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Anti-CDKN2A antibody (Mid-Region) {Biotin} (STJ502025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

$686.55

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