| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat SEPP1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Selenop |
| Gene ID: |
29360 |
| Uniprot ID: |
SEPP1_RAT |
| Specificity: |
This kit recognizes Rat SEPP1 in samples. No significant cross-reactivity or interference between Rat SEPP1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Widely expressed, mainly by the liver. Secreted in plasma. |
| Post Translational Modifications | Isoform Se-P1 contains several disulfide bridges and a selenide-sulfide bond between Sec-59 and Cys-62. These bonds are speculated to serve as redox-active pairs. Phosphorylation sites are present in the extracellular medium. |
| Function | Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. |
| Protein Name | Selenoprotein PSep Cleaved Into - Selenoprotein Se-P10 - Selenoprotein Se-P6 - Selenoprotein Se-P2 - Selenoprotein Se-P1 |
| Database Links | Reactome: R-RNO-114608 |
| Cellular Localisation | SecretedPasses From Plasma Into The Glomerular Filtrate Where It Is Removed By Endocytosis Mediated By Lrp2 In The Proximal Tubule Epithelium |
| Alternative CLIA Names | Selenoprotein P CLIA kitSep Cleaved Into - Selenoprotein Se-P10 - Selenoprotein Se-P6 - Selenoprotein Se-P2 - Selenoprotein Se-P1 CLIA kitSelenop CLIA kitSelp CLIA kitSepp1 CLIA kit |
| Specificity | This kit recognizes Rat SEPP1 in samples. No significant cross-reactivity or interference between Rat SEPP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 84-98 | 91 |
| EDTA plasma(n=8) | 84-97 | 90 |
| Cell culture media(n=8) | 87-99 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.94 | 243.48 | 697.14 | 92.31 | 219.58 | 762.37 |
| Standard deviation | 12.45 | 18.77 | 76.34 | 8.78 | 15.66 | 84.47 |
| CV (%) | 12.98 | 7.71 | 10.95 | 9.51 | 7.13 | 11.08 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
