| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
7.5pg/mL |
| Detection Limit: |
12.5~800pg/mL |
| Short Description: |
This rat CHGA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ChgaUniprot ID=CMGA_RAT" |
| Specificity: |
This kit recognizes Rat CHGA in samples. No significant cross-reactivity or interference between Rat CHGA and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in the brain and adrenal and pituitary glands. |
| Post Translational Modifications | O-glycosylated.contains chondroitin sulfate (CS). CS attachment is pH-dependent, being observed at mildly acidic conditions of pH 5 but not at neutral pH, and promotes self-assembly in vitro. |
| Function | Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Catestatin inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Serpinin regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation. Serpinin and pGlu-serpinin can enhance both myocardial contractility (inotropy) and relaxation (lusitropy) and this cardio-stimulation requires a beta 1-adrenergic receptor/adenylate cyclase/cAMP/PKA pathway. |
| Protein Name | Chromogranin-ACga Cleaved Into - Pancreastatin - Beta-Granin - We-14 - Catestatin - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor |
| Cellular Localisation | Cytoplasmic VesicleSecretory VesicleNeuronal Dense Core VesicleSecretedAssociated With The Secretory Granule Membrane Through Direct Interaction To Scg3 That In Turn Binds To Cholesterol-Enriched Lipid Rafts In Intragranular ConditionsIn Pituitary GonadotropesLocated In Large Secretory GranulesSerpinin: SecretedPyroglutaminated Serpinin Localizes To Secretory Vesicle |
| Alternative CLIA Names | Chromogranin-A CLIA kitCga Cleaved Into - Pancreastatin - Beta-Granin - We-14 - Catestatin - Ge-25 - Serpinin-Rrg - Serpinin - P-Glu Serpinin Precursor CLIA kitChga CLIA kit |
| Specificity | This kit recognizes Rat CHGA in samples. No significant cross-reactivity or interference between Rat CHGA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-99 | 94 |
| EDTA plasma(n=8) | 83-97 | 90 |
| Cell culture media(n=8) | 88-100 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 42.18 | 80.06 | 371.05 | 39.09 | 84.93 | 347.88 |
| Standard deviation | 4.05 | 9.10 | 23.08 | 3.21 | 8.77 | 21.99 |
| CV (%) | 9.60 | 11.37 | 6.22 | 8.21 | 10.33 | 6.32 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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