| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 46.88pg/mL |
| Detection Limit: | 78.13-5000pg/mL |
| Short Description: | This SELP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Selp |
| Gene ID: | 25651 |
| Uniprot ID: | LYAM3_RAT |
| Specificity: | This kit recognizes Rat SELP in samples. No significant crossreactivity or interference between Rat SELP and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3). |
| Protein Name | P-Selectin Cd62 Antigen-Like Family Member P Granule Membrane Protein 140 Gmp-140 Leukocyte-Endothelial Cell Adhesion Molecule 3 Lecam3 Platelet Activation Dependent Granule-External Membrane Protein Padgem Cd Antigen Cd62p |
| Database Links | Reactome: R-RNO-114608 Reactome: -RNO-202733 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative ELISA Names | P-Selectin ELISA kit Cd62 Antigen-Like Family Member P ELISA kit Granule Membrane Protein 140 ELISA kit Gmp-140 ELISA kit Leukocyte-Endothelial Cell Adhesion Molecule 3 ELISA kit Lecam3 ELISA kit Platelet Activation Dependent Granule-External Membrane Protein ELISA kit Padgem ELISA kit Cd Antigen Cd62p ELISA kit Selp ELISA kit |
| Specificity | This kit recognizes Rat SELP in samples. No significant cross-reactivity or interference between Rat SELP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma (n=8) | 92-107 | 98 |
| Cell culture media (n=8) | 90-106 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 272.98 | 776.41 | 1905.72 | 261.66 | 715.67 | 1816.89 |
| Standard deviation | 14.90 | 43.87 | 89.38 | 16.96 | 30.99 | 89.21 |
| CV (%) | 5.46 | 5.65 | 4.69 | 6.48 | 4.33 | 4.91 |
