| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g |
| Immunoreactivity: | Measured in a cell proliferation assay using NFS 60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 2.6â10.6 ng/mL, corresponding to a specific activity of 9.4Ã104~3.85Ã105 units/mg. |
| Immunogen Region: | Ile27-Cys169 |
| Immunogen: | Recombinant Rat IL-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile27-Cys169) of rat IL-3 (Accession #NP_113701.1) fused with a 6ÃHis tag at the C-terminus. |
| Immunogen Sequence: | ISDRGSDAHHLLRTLDCRTI ALEILVKLPYPQVSGLNNSD DKANLRNSTLRRVNLDEFLK SQEEFDSQDTTDIKSKLQKL KCCIPAAASDSVLPGVYNKD LDDFKKKLRFYVIHLKDLQP VSVSRPPQPTSSSDNFRPMT VEC |