Host: |
HEK293 cells |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Determination Method: |
< 0.1 EU/Mu g |
Immunoreactivity: |
Measured in a cell proliferation assay using NFS60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 2.6â10.6 ng/mL, corresponding to a specific activity of 9.4Ã104~3.85Ã105 units/mg. |
Immunogen Region: |
Ile27-Cys169 |
Immunogen: |
Recombinant Rat IL-3 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile27-Cys169) of rat IL-3 (Accession #NP_113701.1) fused with a 6ÃHis tag at the C-terminus. |
Immunogen Sequence: |
ISDRGSDAHHLLRTLDCRTI ALEILVKLPYPQVSGLNNSD DKANLRNSTLRRVNLDEFLK SQEEFDSQDTTDIKSKLQKL KCCIPAAASDSVLPGVYNKD LDDFKKKLRFYVIHLKDLQP VSVSRPPQPTSSSDNFRPMT VEC |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance