Rat IL-18 protein (Recombinant) (C-His) (STJP003102)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Recombinant Rat IL-18 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His37-Ser194) of Rat IL-18 (Accession #NP_062038.1) fused with a His tag at the C-terminus.
General Information
| Short Description | Recombinant-Rat IL-18-C-His protein was developed in hek293 cells using the region His37-Ser194. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% Trehalose is added as protectant before lyophilisation. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method |
Target Information
| Gene Symbol | Il18 |
| Gene ID | 29197 |
| Uniprot ID | IL18_RAT |
| Immunogen | Recombinant Recombinant Rat IL-18 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His37-Ser194) of Rat IL-18 (Accession #NP_062038.1) fused with a His tag at the C-terminus. |
| Immunogen Region | His37-Ser194 |
| Immunogen Sequence | PALGSELKVTTSQVTSWGKS AKNFTCHVTHPPSFNESRTI LVRPVNITEPTLELLHSSCD PNAFHSTIQLYCFIYGHILN DVSVSWLMDDREITDTLAQT VLIKEEGKLASTCSKLNITE QQWMSESTFTCKVTSQGVDY LAHTRRCPDHEPRGVITYLI PPSPLDLYQNGAPKLTCLVV DLESEKNVNVTWNQEKKTSV SASQWYTKHHNNATTSITSI LPVVAKDWIEGYGYQCIVD |
Additional Info
| Post Translational Modifications | The pro-IL-18 precursor is processed by CASP1 to yield its mature, active form. The pro-IL-18 precursor is however not processed by Casp4/Casp11 in rodents. The pro-IL-18 precursor features autoinhibitory interactions between the propeptide and the post-cleavage-site region, preventing recognition by the IL18R1 receptor. Processing by CASP1 induces conformational changes to generate critical receptor-binding sites. The mature form is then secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, cleavage by CASP3 inactivates IL18. |
| Function | Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-18Il-18Interferon Gamma-Inducing FactorIfn-Gamma-Inducing FactorInterleukin-1 GammaIl-1 Gamma |
| Database Links | Reactome: R-RNO-448706Reactome: -RNO-5620971Reactome: -RNO-9012546 |
| Cellular Localisation | CytoplasmCytosolSecretedThe Precursor Is CytosolicIn Response To Inflammasome-Activating SignalsCleaved And SecretedMature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) PoreIn ContrastThe Precursor Form Is Not ReleasedDue To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During MaturationThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative Protein Names | Interleukin-18 proteinIl-18 proteinInterferon Gamma-Inducing Factor proteinIfn-Gamma-Inducing Factor proteinInterleukin-1 Gamma proteinIl-1 Gamma proteinIl18 proteinIgif protein |
Information sourced from Uniprot.org