Rat IL-10 protein (Recombinant) (C-His) (STJP003075)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Rat IL-10 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser19-Asn178) of Rat IL-10 (Accession #NP_036986.2) fused with a His tag at the C-terminus.
General Information
| Short Description | Recombinant-Rat IL-10-C-His protein was developed in hek293 cells using the region Ser19-Asn178. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | <0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Il10 |
| Gene ID | 25325 |
| Uniprot ID | IL10_RAT |
| Immunogen | Recombinant Rat IL-10 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser19-Asn178) of Rat IL-10 (Accession #NP_036986.2) fused with a His tag at the C-terminus. |
| Immunogen Region | Ser19-Asn178 |
| Immunogen Sequence | WPESPKAQASSVPTAQPQAE GSLAKATTAPATTRNTGRGG EEKKKEKEKEEQEERETKTP ECPSHTQPLGVYLLTPAVQD LWLRDKATFTCFVVGSDLKD AHLTWEVAGKVPTGGVEEGL LERHSNGSQSQHSRLTLPRS LWNAGTSVTCTLNHPSLPPQ RLMALREPAAQAPVKLSLNL LASSDPPEAASWLLCEVSGF SPPNILLMWLEDQREVNTSG FAPARPPPQPRSTTFWAWS |
Additional Info
| Function | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Protein Name | Interleukin-10Il-10Cytokine Synthesis Inhibitory FactorCsif |
| Database Links | Reactome: R-RNO-6783783 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-10 proteinIl-10 proteinCytokine Synthesis Inhibitory Factor proteinCsif proteinIl10 proteinIl-10 protein |
Information sourced from Uniprot.org