Post Translational Modifications | Primary antibodies

Western Blot analysis of 1HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ROR2-Ser449 antibody (STJA0009948)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-ROS1-Tyr2114 antibody (STJA0009864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western Blot analysis of Hela using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-ROS1-Tyr2274 antibody (STJ196404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from C6 cells, using Phospho-RPA2-S4/S8 Rabbit pAb (STJ11101136) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 45s.

Anti-Phospho-RPA2-S4/S8 antibody (STJ11101136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€158.13 - €773.13

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Western blot analysis of various cells using Phospho-RPA p32 (S33) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RPA2-Ser33 antibody (1-50 aa) (STJ90636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-RPA2-Ser33 antibody [SRM13700] (STJ11108430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Mouse/Rat

€0.00

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-RPA2-Ser8 antibody (STJ194741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from C6 cells, using Phospho-RPA2-T21 Rabbit mAb (STJ11102614) at 1:1000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-RPA2-T21 antibody [S4MR] (STJ11102614)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€148.13 - €651.88

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Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ug/ml 24h, using RFA2 (Phospho-Thr21) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPA2-Thr21 antibody (10-59 aa) (STJ90903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Anti-Phospho-RPA2-Thr21 antibody [R02-2I5] (STJA0036021)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

€166.88 - €351.88

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Anti-Phospho-Rpb1 CTD-S2/S5-S2/S5 antibody [SRM15121] (STJ11109851)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ChIP/CUT&Tag/ELISA

Reactivity :

Human

€0.00

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Anti-Phospho-Rpb1-Ser2 antibody [PT1003R] (STJA0039928)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€356.25 - €675.00

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Anti-Phospho-Rpb1-Ser5 antibody [PT0923R] (STJA0039821)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Western blot of rat brain lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A phosphorylated at Ser234.

Anti-Phospho-RPH3A-Ser234 antibody (STJA0003761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Rat/Mouse

€610.63

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Immunohistochemistry analysis of paraffin-embedded human brain, using Rabphilin 3A (Phospho-Ser237) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RPH3A-Ser237 antibody (203-252 aa) (STJ91051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from HeLa cells using Phospho-S6 Ribosomal Protein (RPS6) -S235 Rabbit pAb (STJ11105772) at 1:800 dilution. HeLa cells were treated by 20% FBS at 37 °C for 90 minutes after serum starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RPS6-S235 antibody (STJ11105772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-Phospho-RPS6-S235-S235 antibody [SRM15136] (STJ11109866)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S235/236 Rabbit pAb (STJ113489) at 1:1000 dilution. HeLa cells NIH/3T3 cells and PC-12 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6-S235/236 antibody (STJ113489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 Rabbit pAb (STJ113488) at 1:1000 dilution or RPS6 antibody (STJ27852). HeLa and NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6-S240/244 antibody (STJ113488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of lysates from 293 cells treated with serum 10% 15', using S6 Ribosomal Protein (Phospho-Ser235) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser235 antibody (200-249 aa) (STJ90403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of S6 Ribosomal Protein (Phospho-Ser235+Ser236) Antibody. The lane on the right is blocked with the S6 Ribosomal Protein (Phospho-Ser235+Ser236) peptide.

Anti-Phospho-RPS6-Ser235/S236 antibody (200-249 aa) (STJ90992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells, using S6 Ribosomal Protein (Phospho-Ser240) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6-Ser240 antibody (200-249 aa) (STJ91053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-RPS6-Ser240/244 antibody [PT0447R] (STJA0039213)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Western blot of Jurkat cell lysate showing specific immunolabeling of the ~28 kDa rpS6 phosphorylated at Ser244 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-RPS6-Ser244 antibody (STJA0003772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Chicken/Goat/Non-Human Primates/Rat/Xenopus/Zebrafish

€610.63

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Western blot analysis of various lysates using (STJ116387) at 1:1000 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6KA1-S363 antibody (STJ116387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

€158.13 - €773.13

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Western blot analysis of various lysates using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit pAb (STJ113499) at 1:1000 dilution or RPS6KA1 antibody (STJ240768). HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-RPS6KA1-S380 antibody (STJ113499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-S380 Rabbit mAb (STJ11102641) at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-RPS6KA1-S380 antibody [S1MR] (STJ11102641)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Anti-Phospho-RPS6KA1-Ser227 antibody (STJ196410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of 293 cells using Phospho-Rsk-1 (S380) Polyclonal Antibody

Anti-Phospho-RPS6KA1-Ser380 antibody (346-395 aa) (STJ90474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of HeLa, using Phospho-p90Rsk/RSK1/RPS6KA1-T348 antibody (STJ11105866) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-RPS6KA1-T348 antibody (STJ11105866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of lysates from A431 cells, using Phospho-p90Rsk/RSK1/RPS6KA1-T359/S363 Rabbit pAb (STJ113490). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RPS6KA1-T359/S363 antibody (STJ113490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using p90 RSK (Phospho-Thr359+Ser363) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6KA1-Thr359/S363 antibody (331-380 aa) (STJ90825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-RPS6KA1-Thr359/Ser363 antibody (STJ196373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-RPS6KA1-Thr573 antibody (539-588 aa) (STJ91047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-RPS6KA2-Thr353 antibody (STJA0009881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RPS6KA3-Tyr529 antibody (STJA0009989)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of 293 cells using Phospho-MSK2 (T568) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RPS6KA4-Thr568 antibody (531-580 aa) (STJ90952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RPS6KA5-S376 Rabbit pAb (STJ117898) at 1:2000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RPS6KA5-S376 antibody (STJ117898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of 3T3 cells using Phospho-MSK1 (S212) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-RPS6KA5-Ser212 antibody (181-230 aa) (STJ90951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MSK1 (Phospho-Ser360) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6KA5-Ser360 antibody (331-380 aa) (STJ91293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-MSK1 (S376) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-RPS6KA5-Ser376 antibody (343-392 aa) (STJ90677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from C6 cells using Phospho-RPS6KA5-T581 Rabbit pAb (STJ11103736) at1:1000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 60s.

Anti-Phospho-RPS6KA5-T581 antibody (STJ11103736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€158.13 - €773.13

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Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using MSK1 (Phospho-Thr581) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RPS6KA5-Thr581 antibody (551-600 aa) (STJ90695)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-S371 Rabbit pAb (STJ11101156) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RPS6KB1-S371 antibody (STJ11101156)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€158.13 - €773.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-S371 Rabbit pAb (STJ11105784) at 1:400 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RPS6KB1-S371 antibody (STJ11105784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€158.13 - €773.13

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Western blot analysis of COLO cells using Phospho-p70 S6 kinase Alpha (S371) Polyclonal Antibody diluted at 1：500

Anti-Phospho-RPS6KB1-Ser371 antibody (337-386 aa) (STJ90824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of HT-29 serum 20% 15' using p-p70 S6 kinase Alpha (S418) antibody. Antibody was diluted at 1:1000

Anti-Phospho-RPS6KB1-Ser418 antibody (384-433 aa) (STJ91046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of K562 using p-p70 S6 kinase Alpha (S427) antibody.

Anti-Phospho-RPS6KB1-Ser427 antibody (393-442 aa) (STJ91213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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