Post Translational Modifications | Primary antibodies

Immunohistochemistry analysis of paraffin-embedded human brain, using Ras-GRF1 (Phospho-Ser916) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Ras-GRF1-Ser916 antibody (882-931 aa) (STJ91135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-RB-S795-S795 antibody [SRM15125] (STJ11109855)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

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Anti-Phospho-Rb-Ser608 antibody [R02-6C5] (STJA0035092)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

$184.23 - $388.47

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Anti-Phospho-Rb-Ser780 antibody [PT0992R] (STJA0039936)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$393.30 - $745.20

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Anti-Phospho-Rb-Ser780 antibody [R01-8H7] (STJA0034802)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Anti-Phospho-Rb-Ser780 antibody [R04-5B7] (STJA0035093)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

$184.23 - $388.47

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Anti-Phospho-Rb-Ser807 antibody (STJA0037464)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-Rb-Ser807 antibody [R04-3H1] (STJA0035938)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$184.23 - $388.47

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Anti-Phospho-Rb-Ser807/811 antibody [PT0996R] (STJA0040071)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-Phospho-Rb-Ser811 antibody [R01-2G6] (STJA0034803)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Hamster

$184.23 - $388.47

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Anti-Phospho-Rb-Thr252 antibody [R03-8H1] (STJA0035094)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

$184.23 - $388.47

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Anti-Phospho-Rb-Thr356 antibody [R04-1C4] (STJA0035095)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

$184.23 - $388.47

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Western blot analysis of lysates from Jurkat cells, using Phospho-RB-S608 Rabbit pAb (STJ117879) at 1:2000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RB1-S608 antibody (STJ117879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RB-S780 Rabbit pAb (STJ22373) at dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RB1-S780 antibody (STJ22373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of C6, using Phospho-RB-S780 antibody (STJ11105845) at 1:10000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Phospho-RB1-S780 antibody [S5845RM] (STJ11105845)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of lysates from C6 cells, using Phospho-RB-S780 Rabbit monoclonal antibody (STJ11105875) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-RB1-S780 antibody [S5875RM] (STJ11105875)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Western blot analysis of lysates from C6 cells, using Phospho-RB-S780 Rabbit mAb (STJ11102555) at 1:1000 dilution. C6 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-RB1-S780 antibody [S5MR] (STJ11102555)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Western blot analysis of lysates from 293 cells, using Phospho-RB-S795 Rabbit pAb (STJ22369). 293 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RB1-S795 antibody (STJ22369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HT-29 cells, using Phospho-RB-S807/811 Rabbit pAb (STJ114837) at 1:1000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-RB1-S807/811 antibody (STJ114837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells, using Phospho-RB-S811 Rabbit pAb (STJ22370) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RB1-S811 antibody (STJ22370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.57 - $853.53

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Immunofluorescence analysis of HeLa cells, using Retinoblastoma (Phospho-Ser249) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RB1-Ser249 antibody (221-270 aa) (STJ91344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30', using Retinoblastoma (Phospho-Ser608) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RB1-Ser608 antibody (581-630 aa) (STJ90934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunofluorescence analysis of Hela cell. 1, Rb (phospho Ser780) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-RB1-Ser780 antibody (751-800 aa) (STJ90400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RB1-Ser788 antibody (756-805 aa) (STJ91193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Retinoblastoma (Phospho-Ser795) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RB1-Ser795 antibody (761-810 aa) (STJ90401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of various cells using Phospho-Rb (S807) Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RB1-Ser807 antibody (781-830 aa) (STJ90402)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RB1-Ser807/811 antibody (STJ196398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from K562 cells, using Retinoblastoma (Phospho-Ser811) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-RB1-Ser811 antibody (771-820 aa) (STJ90699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RB1-Thr252 antibody (STJA0009842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemistry analysis of paraffin-embedded human testis, using Retinoblastoma (Phospho-Thr821) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RB1-Thr821 antibody (791-840 aa) (STJ91297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of RAT-musle using p-Rb (T826) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-RB1-Thr826 antibody (601-650 aa) (STJ90716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Anti-Phospho-Rb2 p130-Ser952 antibody [R02-5D6] (STJA0034805)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$184.23 - $388.47

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Anti-Phospho-Rb2 p130-Thr986 antibody [R06-9B3] (STJA0033909)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

$184.23 - $388.47

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Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Ser326 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-RBBP8-Ser326 antibody (STJA0003827)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

$674.13

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Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Ser327 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-RBBP8-Ser327 antibody (STJA0003828)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

$674.13

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Anti-Phospho-RBBP8-Ser327 antibody (STJA0009814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western Blot analysis of HepG2 cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RBBP8-Ser664 antibody (STJA0010034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot of human T47D cell lysate showing specific immunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-RBBP8-Thr847 antibody (STJA0003829)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Canine/Chicken/Finch/Goat/Guinea Pig/Non-Human Primates/Mouse/Sheep

$674.13

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Immunohistochemistry analysis of paraffin-embedded human lymph node, using RBL1 (Phospho-Thr369) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RBL1-Thr369 antibody (335-384 aa) (STJ91140)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western Blot analysis of 1 HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RBL2-Ser639 antibody (STJA0010066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-RBL2-Ser952 antibody (STJA0009825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western Blot analysis of 1 HeLa cell, 22 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-RBL2-Thr642 antibody (STJA0010062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$67.62 - $360.18

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-RCC1-Ser11 antibody (STJ194740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-Phospho-REL-Ser492/460 antibody (STJA0010025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$67.62 - $360.18

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Western blot analysis of 3T3 cells using Phospho-c-Rel (S503) Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-REL-Ser503 antibody (470-519 aa) (STJ91012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S311 Rabbit pAb (STJ22380) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RELA-S311 antibody (STJ22380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells, using Phospho-NF-kB p65/RelA-S468 Rabbit pAb (STJ22381) at 1:400 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-RELA-S468 antibody (STJ22381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.57 - $853.53

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Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S529 Rabbit pAb (STJ11101077) at 1:500 dilution incubated overnight at 4 °C. HeLa cells were treated by TNF-Alpha (100 ng/ml) and Calyculin A (50 nM) at 37 °C for 10 minutes. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 60s.

Anti-Phospho-RELA-S529 antibody (STJ11101077)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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