Rabbit Monoclonal Antibodies | St John's Laboratory

Anti-Phospho-PKC gamma-Thr514 antibody [R02-4F3] (STJA0034729)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

€168.60 - €370.20

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Anti-Phospho-PKC zeta-Thr560 antibody [R06-9T9] (STJA0036100)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Anti-Phospho-PKC zeta/lambda-Thr410/Thr412 antibody [R03-3D0] (STJA0035087)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse

€160.20 - €337.80

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Anti-Phospho-PKC-Ser660 antibody [R08-0H5] (STJA0035084)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

€160.20 - €337.80

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Anti-Phospho-PKR-Thr446 antibody [R03-5B4] (STJA0035856)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ChIP

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-PKR-Thr451 antibody [R08-2G5] (STJA0034735)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€168.60 - €370.20

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-PLC gamma 1 (PLCG1) -S1248 Rabbit mAb (STJ11107733) at 1:3000 dilution incubated overnight at 4C. NIH/3T3 cells were treated by PDGF-BB (100 ng/ml) for 30 minutes. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.

Anti-Phospho-PLC gamma 1-S1248 antibody [SRM5544] (STJ11107733)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Anti-Phospho-PLC Gamma 1-Tyr783 antibody [PT0758R] (STJA0039822)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Anti-Phospho-PLC gamma 2 (PLCG2)-Y1217-Y1217 antibody [SRM15154] (STJ11109884)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€0.00

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Anti-Phospho-PLC gamma 2 (PLCG2)-Y759 antibody [SRM13705] (STJ11108435)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€0.00

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Anti-Phospho-PLC Gamma 2-Tyr1217 antibody [PT1383R] (STJA0040084)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

€180.00 - €648.00

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Western blot analysis of various lysates, using Phospho-PLC gamma 1 (PLCG1) -Y783 antibody (STJ11105829) at1:2000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-PLCG1-Y783 antibody [S5829RM] (STJ11105829)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€142.20 - €625.80

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Western blot analysis of lysates from HT-29 cells, using Phospho-PLK1-T210 (STJ11102606) at 1:1000 dilution. HT-29 cells were treated by nocodazole (100 ng/mL) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-PLK1-T210 antibody [S6MR] (STJ11102606)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-Phospho-PLK1-Thr210 antibody [R08-3V9] (STJA0036131)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-PML Protein-Ser518 antibody [R01-3D1] (STJA0035088)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

€160.20 - €337.80

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Chromatin immunoprecipitations were performed with cross-linked chromatin from 293F cells and Phospho-POLR2A-S2 (STJ11102585). The ChIP sequencing results indicate the enrichment pattern of Phospho-POLR2A-S2 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Phospho-POLR2A-S2 antibody [S5MR] (STJ11102585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Western blot analysis of various lysates using Phospho-POLR2A CTD-S5 Rabbit mAb (STJ11102586) at 1:1000 dilution. MCF7 cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours or treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or treated by CIP (20uL/400ul) at 37 °C for 1 hour. C2C12 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-POLR2A-S5 antibody [S6MR] (STJ11102586)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Western blot analysis of various lysates, using Phospho-POLR2A-T4 monoclonal antibody antibody (STJ11105859) at 1:6000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-POLR2A-T4 antibody [S5859RM] (STJ11105859)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Anti-Phospho-PP2A alpha-Tyr307 antibody [R07-9C6] (STJA0035755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Anti-Phospho-PPP1CA-T320 antibody [SRM13694] (STJ11108424)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-Phospho-PPP1CA-T320 antibody [SRM13710] (STJ11108440)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PP2A Catalytic Alpha-Y307 Rabbit mAb (STJ11102616) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-PPP2CA-Y307 antibody [S6MR] (STJ11102616)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€142.20 - €625.80

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Anti-Phospho-PRAS40-Thr246 antibody [PT0756R] (STJA0039222)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Anti-Phospho-PRC1-Thr481 antibody [R06-2A3] (STJA0034750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-Presenilin 1-Ser310 antibody [R07-6E3] (STJA0033903)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€168.60 - €370.20

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Anti-Phospho-Presenilin 2-Ser330 antibody [R01-5C9] (STJA0033916)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of lysates from C2C12 cells, using Phospho-AMPK Alpha 1-S496 Rabbit mAb (STJ11102591) at 1:1000 dilution. C2C12 cells were treated by oligomycin (0. 5 Mu M) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-PRKAA1-S496 antibody [S1MR] (STJ11102591)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€142.20 - €625.80

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Western blot analysis of lysates from 293T cells, using Phospho-PKA RII Alpha (PRKAR2A) -S99 Rabbit mAb (STJ11102613) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PRKAR2A-S99 antibody [S3MR] (STJ11102613)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€142.20 - €625.80

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Western blot analysis of 293T cells, using Phospho-PKC alpha-T638 antibody (STJ11105853) at 1:7000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-PRKCA-T638 antibody [S5853RM] (STJ11105853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Western blot analysis of lysates from C2C12 cells, using Phospho-PKC alpha-T638 Rabbit mAb (STJ11102617) at 1:1000 dilution. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-PRKCA-T638 antibody [S7MR] (STJ11102617)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€142.20 - €625.80

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Western blot analysis of various lysates using Phospho-PKC zeta-T560 Rabbit mAb (STJ11102642) at 1:1000 dilution. Both 293T cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-PRKCZ-T560 antibody [S2MR] (STJ11102642)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€142.20 - €625.80

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Anti-Phospho-PRKD2-Ser876 antibody [R06-4D1] (STJA0034733)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-Progesterone Receptor-Ser190 antibody [R08-5E7] (STJA0034756)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human

€160.20 - €337.80

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Western blot analysis of lysates from 293T cells, using Phospho-PTEN-S380 Rabbit mAb (STJ11102587) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-PTEN-S380 antibody [S7MR] (STJ11102587)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-Phospho-PTEN-S380/T382/T383-S380/T382/T383 antibody [SRM15120] (STJ11109850)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-Phospho-PTEN-Ser380 antibody [R08-7W6] (STJA0036134)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of A-549, using Phospho-FAK-Y397 Rabbit monoclonal antibody (STJ11105895) at 1:4000 dilution. A-549 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PTK2-Y397 antibody [S5895RM] (STJ11105895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€142.20 - €625.80

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Anti-Phospho-PTP1B-Ser352 antibody [R04-1F2] (STJA0033915)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-PTP1B-Ser378 antibody [R08-5F7] (STJA0035089)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-PTS-Ser19 antibody [R05-7G8] (STJA0035090)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

€160.20 - €337.80

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Western blot analysis of various lysates using Phospho-Paxillin-Y118 Rabbit mAb (STJ11102648) at 1:1000 dilution. A-431 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by pervanadate (1 mM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-PXN-Y118 antibody [S8MR] (STJ11102648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Anti-Phospho-Rac1/CDC42-Ser71 antibody [R09-6M1] (STJA0036206)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-Rad17-Ser656 antibody [R08-3F4] (STJA0035091)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

€160.20 - €337.80

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Anti-Phospho-Raf-1-Ser259 antibody [PT1225R] (STJA0040173)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-Phospho-Raf-1-Ser338 antibody [PT0881R] (STJA0039934)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Anti-Phospho-Raf-B-Thr401 antibody [PT0329R] (STJA0039293)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of various lysates using Phospho-Raf1-S259 Rabbit mAb (STJ11102598) at 1:1000 dilution. Both 293T cells and C6 cells were treated by Forskolin (10 uM) at 37 °C for 1 hour after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-RAF1-S259 antibody [S8MR] (STJ11102598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€142.20 - €625.80

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Western blot analysis of lysates from C6 cells, using Phospho-Raf1-S621 Rabbit mAb (STJ11102597) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-RAF1-S621 antibody [S7MR] (STJ11102597)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€142.20 - €625.80

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