Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CD84 antibody (STJ11103482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD84 antibody (246-345) [S2MR] (STJ11103482)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-CD84 antibody [152-1D5] (STJ16103081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IF/IP/WB

Reactivity :

Human

£391.50

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Anti-CD84 antibody [153-4D9] (STJ16100008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

£308.50 - £1,349.50

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Anti-CD84 antibody [153-4D9] (STJ16101951)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IF/WB

Reactivity :

Human

£391.50

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Anti-CD84 antibody [R85-3Z-9] (STJA0035549)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£133.50 - £281.50

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Anti-CD84 antibody [SRM13390] (STJ11108120)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Human

£0.00

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Anti-CD85j antibody [GHI/75] (STJ16103326)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Human

£419.50

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Western blot analysis of extracts of various cell lines, using CD86 antibody (STJ11101564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD86 antibody (1-100) [S4MR] (STJ11101564)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of Raji cells using CD86 Rabbit polyclonal antibody (STJ23014) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD86 antibody (25-133) (STJ23014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 3 Mu g CD86 antibody (STJ119185). Western blot was performed from the immunoprecipitate using CD86 antibody (STJ119185) at a dilution of 1:1000.

Anti-CD86 antibody (25-329) (STJ119185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot with Anti-CD86 Antibody (STJ500486) with peptide on at 1:500 dilution in buffer.

Anti-CD86 antibody (250-329) (STJ500486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£400.50

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Anti-CD86 antibody (250-329) {Biotin} (STJ500487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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Anti-CD86 antibody (250-329) {FITC} (STJ500488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£497.50

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Anti-CD86 antibody (STJA0010129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis using CD86 monoclonal antibody against L1210 (1) and MOLT-4 (2) cell lysate.

Anti-CD86 antibody [1B3] (STJ97939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-CD86 antibody [BU63] (STJ16103327)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-F

Reactivity :

Human

£447.50

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Anti-CD86 antibody [BU63] (STJA0001270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/Block/IP

Reactivity :

Human

£390.50 - £12,757.50

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Anti-CD86 antibody [PT0048R] (STJA0039758)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse

£285.00 - £540.00

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Anti-CD86 antibody [R04-2A6] (STJA0035708)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Rat

£133.50 - £281.50

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Anti-CD87/PLAUR/uPAR antibody (Leu23-Arg303) [1A450] (STJA0022397)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD87/PLAUR/uPAR antibody (Leu23-Arg303) [1A451] (STJA0022398)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD87/PLAUR/uPAR antibody [1A437] (STJA0022285)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IF/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD87/PLAUR/uPAR antibody [1A438] (STJA0022286)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IF/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD87/PLAUR/uPAR antibody [1A439] (STJA0022287)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IF/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD88 antibody [P12/1] (STJ16100547)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC/IHC-F

Reactivity :

Human/Rhesus

£985.50

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Anti-CD88 antibody [S5/1] (STJ16100838)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Rabbit/Ferret/Bovine/Mustelid/Mink

£985.50

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Anti-CD88 antibody [S5/1] (STJ16103329)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB/IHC-P

Reactivity :

Rabbit/Ferret/Cow/Human

£419.50

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Anti-CD89 antibody [PT0770R] (STJA0039331)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ICC/FC/IP

Reactivity :

Human

£150.00 - £540.00

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Anti-CD89 antibody [R02-3E5] (STJA0036941)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human

£133.50 - £281.50

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Anti-CD89 antibody [SRM13288] (STJ11108018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£0.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD8 a (ABT155) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MOLT-4Predicted band size: 26kDaObserved band size: 35kDa

Anti-CD8A antibody (100-235 aa) [ABT155] (STJ197126)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£180.00 - £261.00

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Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD8 a (ABT455) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Daudi Lane 2: Raji Lane 3: MOLT-4Predicted band size: 26kDaObserved band size: 24kDa

Anti-CD8A antibody (100-235 aa) [ABT304] (STJ197218)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£180.00 - £261.00

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Western blot analysis of extracts of various cell lines, using CD8A rabbit monoclonal antibody (STJ11101251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CD8A antibody (136-235) [S1MR] (STJ11101251)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Anti-CD8A antibody (22-182 aa) [SRM5122] (STJ11106940)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using CD8A Rabbit polyclonal antibody (STJ114922) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD8A antibody (22-235) (STJ114922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various lysates, using CD8A Rabbit polyclonal antibody (STJ11104719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD8A antibody (STJ11104719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Anti-CD8A antibody (STJA0010229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-Cd8a antibody [53-6.7] (STJA0001670)

Clonality :

Monoclonal

Host :

Rat

Applications :

Block/Depletion/FC/WB/IF/IP

Reactivity :

Mouse

£185.50 - £2,845.50

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Anti-Cd8a antibody [53-6.7] (STJA0001671)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/WB/IF/IP

Reactivity :

Mouse

£180.50 - £2,642.50

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Anti-CD8A antibody [R4E25] (STJA0032134)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

ELISA/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£406.50

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Anti-CD8a/Lyt2 antibody [53-6.72] (STJA0027418)

Clonality :

Monoclonal

Host :

Rat

Applications :

Blocking/FC/IF/WB

Reactivity :

Mouse

£112.50 - £203.50

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Anti-CD8a/Lyt2 antibody [53-6.72] {APC} (STJA0028965)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/IF/WB

Reactivity :

Mouse

£106.50 - £246.50

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Anti-CD8a/Lyt2 antibody [53-6.72] {PerCP} (STJA0029931)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/IF/WB

Reactivity :

Mouse

£119.50 - £231.50

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Anti-CD8a/Lyt2 antibody [53-6.72] {PE} (STJA0030897)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/IF/WB

Reactivity :

Mouse

£99.50 - £246.50

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Anti-CD8a/Lyt2 antibody [53-672] {FITC} (STJA0027999)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/IF/WB

Reactivity :

Mouse

£118.50 - £203.50

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD8B using CD8B Rabbit polyclonal antibody (STJ23016) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD8B antibody (22-170) (STJ23016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD8B antibody (STJ192945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-CD8b/Lyt3 antibody [35.17.2#] (STJA0027419)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/IF/IP/RIA/WB

Reactivity :

Mouse

£231.50 - £300.50

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