Western Blot Antibodies | St John's Laboratory

STJ70923 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ACSL5 antibody (C-Term) (STJ70923)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

€390.63

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Western blot analysis of lysates from Rat brain, using ACSL6 Rabbit pAb (STJ110272) at 1:2000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-ACSL6 antibody (383-622) (STJ110272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using ACSL6 Polyclonal Antibody diluted at 1：1000

Anti-ACSL6 antibody (499-548 aa) (STJ91457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using ACSM2A Rabbit polyclonal antibody (STJ117758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ACSM2A antibody (418-577) (STJ117758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Anti-ACSM2A antibody (STJA0009557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€60.00 - €315.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACSM2B antibody (264-314 aa) (STJ195962)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-ACSM2B antibody (STJ11107009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-ACSM3 antibody (239-438 aa) (STJ11107489)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from Mouse kidney using ACSM3 Rabbit polyclonal antibody (STJ11105568) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-ACSM3 antibody (28-130) (STJ11105568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€140.63 - €655.63

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Western blot analysis using ACSM3 monoclonal antibody against K562 cell lysate.

Anti-ACSM3 antibody (C-Term) (STJ98459)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow/Pig

€180.00 - €435.00

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ACSM5 using ACSM5 Rabbit polyclonal antibody (STJ11105444) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.

Anti-ACSM5 antibody (27-130) (STJ11105444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-ACSM5 antibody (STJA0008853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of L929 cells using ACSS1 Rabbit polyclonal antibody (STJ117203) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACSS1 antibody (340-689) (STJ117203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using ACSS1 Polyclonal Antibody

Anti-ACSS1 antibody (611-660 aa) (STJ91458)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of HEPG2 cells using Antibody diluted at 800. Secondary antibody was diluted at 1:20000

Anti-ACSS1 antibody (620-689 aa) (STJ98641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-ACSS2 antibody (40-120 aa) (STJ190496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using ACSS2 antibody (STJ115557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ACSS2 antibody (462-701) (STJ115557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€140.63 - €655.63

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Immunofluorescence analysis of NIH-3T3 cells using ACSS2 Rabbit polyclonal antibody (STJ28555) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACSS2 antibody (462-701) (STJ28555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of HepG2 cells, using ACSS2 Rabbit monoclonal antibody (STJ11101408) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ACSS2 antibody (50-150) [S8MR] (STJ11101408)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of various lysates using ACSS3 Rabbit polyclonal antibody (STJ115726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACSS3 antibody (30-280) (STJ115726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Anti-ACSS3 antibody (STJA0007755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Confocal imaging of paraffin-embedded Mouse skeletal muscle tissue using Alpha-Actin-1 (ACTA1) Rabbit monoclonal antibody (STJ11101812, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ACTA1 antibody (1-100) [S2MR] (STJ11101812)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of various cells using Actin Alpha 1 Polyclonal Antibody diluted at 1：500

Anti-ACTA1 antibody (1-50 aa) (STJ91462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Alpha-Actin-1 (ACTA1) Rabbit monoclonal antibody (STJ11101805) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACTA1 antibody (250-350) [S5MR] (STJ11101805)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunocytochemistry analysis of muscle Actin (green) in Hela using muscle Actin antibody, and DAPI (blue).

Anti-ACTA1 antibody (363-376 aa) [R03-5I2] (STJA0011635)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat

€308.13

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Western blot analysis of MFC cells using Alpha-SMA Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ACTA1 antibody (C-Term) (STJ96485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Mouse Skeletal Muscle Tissue using Muscle Actin Mouse mAb diluted at 1:200.

Anti-ACTA1 antibody [3E9] (STJ97754)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, Alpha skeletal muscle actin monoclonal antibody (4B11) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-ACTA1 antibody [4B11] (STJ97051)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~42 kDa actin protein.

Anti-ACTA1 antibody [C4] (STJA0003575)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

All

€504.38

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Immunofluorescence analysis of paraffin-embedded mouse large intestine using Alpha-Smooth Muscle Actin (ACTA2) Rabbit polyclonal antibody (STJ29391) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACTA2 antibody (1-100) (STJ29391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of paraffin-embedded mouse colon using Alpha-Smooth Muscle Actin (ACTA2) Rabbit polyclonal antibody (STJ114915) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACTA2 antibody (1-377) (STJ114915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from 1) Jurkat, 2) K562, 3) HuvEC cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-ACTA2 antibody (84-134 aa) (STJ96418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain using Alpha-SMA monoclonal antibody.

Anti-ACTA2 antibody [1E12] (STJ97381)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis using ACTA2 monoclonal antibody against HeLa (1) , A431 (2) , Jurkat (3) , K562 (4) , HEK293 (5) , HepG2 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-ACTA2 antibody [4A4] (STJ97813)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€180.00 - €435.00

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Immunohistochemistry with Anti-ACTB Antibody (STJ500039) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section. Right slide is negative control.

Anti-ACTB antibody (1-50) (STJ500039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€534.38

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Anti-ACTB antibody (1-50) {Biotin} (STJ500043)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€660.63

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Anti-ACTB antibody (1-50) {FITC} (STJ500044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

€660.63

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Western blot analysis of customer's lysis using Actin Beta antibody. Antibody was diluted at 1:2000

Anti-ACTB antibody (1-80 aa) (STJ91464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Globefish/Cow/Hamster/Pig/Sheep/Cat/Pig/Dog/Sheep

€60.00 - €315.00

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Anti-beta-Actin antibody at 1:500 dilution; lysates at 100 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ACTB antibody (100aa N-Term) (STJ140081)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€126.88 - €493.13

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Anti-ACTB antibody (C-Term) [M202] (STJA0005244)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat

€399.38

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Anti-ACTB antibody (N-Term) (STJA0005241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC/IP

Reactivity :

Chicken/Human/Mouse/Rat

€399.38

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Immunofluorescence analysis of Hela cell. 1, Cyclin A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-actin monoclonal antibody (5B7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ACTB antibody [5B7] (STJ96930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect/Pig/Lenok

€60.00 - €315.00

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Anti-ACTB antibody [5B7] {AbFluor™ 350} (STJA0006194)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 405} (STJA0006195)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 488} (STJA0006196)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 555} (STJA0006197)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 594} (STJA0006198)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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Anti-ACTB antibody [5B7] {AbFluor™ 647} (STJA0006199)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Insect

€180.00 - €300.00

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