Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from Jurkat cells, treated with UV 15', using CD3 zeta Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CD247 antibody (111-160 aa) (STJ92112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$67.62 - $360.18

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Immunofluorescence analysis of A549 cells using CD3H antibody (STJ22982). Blue: DAPI for nuclear staining.

Anti-CD247 antibody (50-164) (STJ22982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Flow cytometry:1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3H Rabbit monoclonal antibody (STJ11101284, 10 Mu g/mL, orange line) or Rabbit IgG isotype control ( 10 Mu g/mL, blue line) , followed by FITC conjugated goat anti-rabbit polyclonal antibody (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Anti-CD247 antibody (65-164) [S4MR] (STJ11101284)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human

$174.57 - $765.21

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Western blot analysis using CD247 monoclonal antibody against CD247-hIgGFc transfected HEK293 cell lysate.

Anti-CD247 antibody [4B10] (STJ97916)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Western blot analysis of extracts of various cell lines, using CD248 antibody (STJ114576) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from HeLa cells, using CD248 Rabbit polyclonal antibody (STJ114579) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CD248 antibody (18-150) (STJ114579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$152.49 - $697.59

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Western blot analysis of HepG2 cells using CD248 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD248 antibody (341-390 aa) (STJ96632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Anti-CD25 antibody [SRM13386] (STJ11108116)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse

$0.00

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Anti-CD25 antibody [SRM14597] (STJ11109327)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$0.00

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Anti-CD25/IL2RA antibody [143-13] (STJ16102559)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/FUNC/WB/IF/IHC

Reactivity :

Human

$425.73 - $1,862.31

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Anti-CD26 antibody [202-36] (STJ16102562)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human/Rat

$425.73 - $1,862.31

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Anti-CD26 antibody [202.36] (STJ16100026)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IHC-F/IF/WB

Reactivity :

Human/Rat

$520.95

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Anti-CD26 antibody [5+00E+08] (STJ16100784)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/ELISA/IP/WB

Reactivity :

Rat

$914.25

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Anti-CD26 antibody [PT1307R] (STJA0040113)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Anti-CD26/DPP4 antibody [K9/9] (STJA0027434)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC/IP/RIA/WB

Reactivity :

Mouse/Rat

$319.47 - $414.69

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Anti-CD26/DPP4 antibody [K9/9] {APC} (STJA0029342)

Clonality :

Monoclonal

Host :

Rat

Applications :

IF/WB

Reactivity :

Mouse/Rat

$389.85 - $505.77

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Anti-CD26/DPP4 antibody [K9/9] {FITC} (STJA0028376)

Clonality :

Monoclonal

Host :

Rat

Applications :

IF/WB

Reactivity :

Mouse/Rat

$319.47 - $411.93

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Anti-CD26/DPP4 antibody [K9/9] {PerCP} (STJA0030308)

Clonality :

Monoclonal

Host :

Rat

Applications :

IF/WB

Reactivity :

Mouse/Rat

$366.39 - $475.41

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Anti-CD26/DPP4 antibody [K9/9] {PE} (STJA0031274)

Clonality :

Monoclonal

Host :

Rat

Applications :

IF/WB

Reactivity :

Mouse/Rat

$389.85 - $505.77

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Anti-CD26/DPP4 antibody [SRM13292] (STJ11108022)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/FC/ELISA

Reactivity :

Human

$0.00

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Anti-CD261 antibody [B-N28] (STJ400461)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

$0.00

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Anti-CD262/TNFRSF10B/TRAIL-R2/DR5 antibody [SAA2021] (STJA0020568)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

$293.25 - $1,634.61

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Anti-CD263 antibody [B-H47] (STJ400462)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$0.00

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Anti-CD264 antibody [B-P30] (STJ400472)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human

$0.00

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Confocal imaging of human tonsil using CD27 Rabbit monoclonal antibody (STJ11101350, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol.

Anti-CD27 antibody (1-100) [S0MR] (STJ11101350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

$163.53 - $719.67

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD27 antibody (1-80 aa) (STJ191672)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$67.62 - $360.18

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Whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-CD27 (PT1818) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: K562Predicted band size: 29kDaObserved band size: 40kDa

Anti-CD27 antibody (160-260) [PT1818] (STJA0006232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$67.62 - $360.18

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STJ73070 (0. 3µg/ml) staining of Human Spleen (A) , Tonsil (B) and Thymus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD27 antibody (161-175) (STJ73070)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$431.25

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Western blot analysis of lysates from Mouse lung, using CD27 Rabbit polyclonal antibody (STJ118100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD27 antibody (20-191) (STJ118100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$152.49 - $697.59

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Western blot analysis of extracts of Raji cells, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD27 antibody (20-191) (STJ22984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-CD27 antibody [203-6] (STJ16101136)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

$425.73 - $1,862.31

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Anti-CD27 antibody [5F24] (STJA0027858)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$319.47 - $414.69

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Anti-CD27 antibody [5F24] {APC} (STJA0029338)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$389.85 - $505.77

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Anti-CD27 antibody [5F24] {FITC} (STJA0028372)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$319.47 - $411.93

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Anti-CD27 antibody [5F24] {PerCP} (STJA0030304)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$366.39 - $475.41

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Anti-CD27 antibody [5F24] {PE} (STJA0031270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB

Reactivity :

Human

$389.85 - $505.77

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Anti-CD27 antibody [PT1331R] (STJA0040116)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$207.00 - $745.20

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Flow cytometry:1X10^6 293F (Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD271/NGFR Rabbit monoclonal antibody (STJ11105254, 2 Mu g/mL, right).

Anti-CD271 antibody (32-254) [S5254RM] (STJ11105254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse/Rat

$174.57 - $765.21

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD272 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-CD272 antibody (30-183 aa) [R09-4A1] (STJA0011098)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

$340.17

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Anti-CD272/BTLA antibody [SRM13405] (STJ11108135)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Mouse

$0.00

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Western blot analysis of lysates from Mouse thymus, using CD274 Rabbit monoclonal antibody (STJ11105258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (19-235) [S5258RM] (STJ11105258)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$163.53 - $719.67

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ113753) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (19-238) (STJ113753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using CD274 antibody (STJ11103333) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD274 antibody (19-239) (STJ11103333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ22985) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD274 antibody (190-290) (STJ22985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunohistochemistry of baboon spleen with Anti-CD274 primary antibody (STJ502399) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD274 antibody (80-130) (STJ502399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$552.69

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Anti-CD274 antibody (80-130) {Biotin} (STJ502400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$686.55

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Anti-CD274 antibody (80-130) {FITC} (STJ502401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$686.55

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274/CD274 Rabbit monoclonal antibody (STJ11103966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (ECD) [S3966RM] (STJ11103966)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$174.57 - $765.21

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