Immunofluorescence Antibodies | St John's Laboratory

IP antibody use：5ug His Mouse IgG1 per ml Lysate, WB 1:3000, 1. untransfected 293 cell lysate, 2. transfected 293 cell lysate with His-tag fusion protein, 3. IP (untransfected 293+anti-His mAb+ Protein G agarose) , 4. IP (transfected 293+ normal Mouse IgG+Protein G agarose) , 5. IP (transfected 293+anti-His mAb+ Protein G agarose) , 6. Recombinant protein (E.coli)

Anti-His-Tag antibody [4E6] (STJ96906)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£48.00 - £252.00

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Anti-His-Tag antibody [4E6] {AbFluor™ 350} (STJA0006079)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {AbFluor™ 405} (STJA0006080)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {AbFluor™ 488} (STJA0006081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {AbFluor™ 555} (STJA0006082)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {AbFluor™ 594} (STJA0006083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {AbFluor™ 647} (STJA0006084)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {AbFluor™ 680} (STJA0006085)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {Cy3} (STJA0006086)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {Cy5} (STJA0006087)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {FITC} (STJA0006088)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Anti-His-Tag antibody [4E6] {HRP} (STJA0006105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/IF

Reactivity :

Species independent

£144.00 - £240.00

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Western blot analysis of lysates from His Protein, using Rabbit anti His-tag mAb (STJ11103712) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-His-Tag antibody [S2MR] (STJ11103712)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

£207.50 - £392.50

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Western blot analysis of various lysates, using His-Tag mAb (STJ11103695) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-His-Tag antibody [S5MM] (STJ11103695)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

£59.50 - £249.50

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Anti-His-Tag antibody [SMM] (STJ11107566)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

£135.50 - £249.50

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Anti-His-tag antibody [SRM] (STJ11107582)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Species independent

£135.50 - £249.50

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Western blot analysis of various lysates using HIST1H2BG Rabbit pAb (STJ11107549) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-HIST1H2BG antibody (1-126 aa) (STJ11107549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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STJ70765 (4µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-Histamine Receptor H1 antibody (C-Term) (STJ70765)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ70766 (1µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Histamine Receptor H2 antibody (C-Term) (STJ70766)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ70893 (0. 1µg/ml) staining of Human Duodenum (A) and Lymph Node (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Histone Deacetylase 1 antibody (C-Term) (STJ70893)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/ChIP

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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Immunofluorescence analysis of Hela cell. 1, Histone H1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Histone H1 antibody (1-50 aa) (STJ93515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Histone H1t antibody (STJ11100542) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Histone H1 antibody (110-209) (STJ11100542)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Anti-Histone H1 antibody [AE-4] (STJ16100269)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human/Mouse/Rat

£287.50 - £1,324.50

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Confocal imaging of U-2 OS cells using Histone H1. 2 Rabbit monoclonal antibody (STJ11102702, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-Histone H1.2 antibody (1-100) [S2MR] (STJ11102702)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £574.50

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Immunocytochemistry analysis of Histone H1. 3 (green) in Hela using Histone H1. 3 antibody, and DAPI (blue)

Anti-Histone H1.3 antibody (5-17 aa) [R09-8H7] (STJA0011419)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£246.50

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Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (STJ29383) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Histone H2A antibody (1-143) (STJ29383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Western blot analysis of various lysates using Histone H2AX Rabbit pAb (STJ113097) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Histone H2A antibody (50-143) (STJ113097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Histone H2A Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Histone H2A antibody (Internal) (STJ96865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Histone H3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Histone H3 antibody (1-50 aa) (STJ93528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Cat/Rice

£48.00 - £252.00

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Anti-Histone H3 antibody (36-136) (STJ23980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, Amyloid-Beta Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Histone H3 antibody [1G1] (STJ96940)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/Yeast/A.thaliana

£48.00 - £252.00

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Anti-Histone H3 antibody [SM0412] (STJA0010412)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunofluorescence analysis of NIH/3T3 cells using Histone H4 Rabbit polyclonal antibody (STJ119273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Histone H4 antibody (1-100) (STJ119273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£112.50 - £524.50

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Immunocytochemistry analysis of Histone H4 (green) in Hela using Histone H4 antibody, and DAPI (blue).

Anti-Histone H4 antibody (51-70 aa) [R05-8H9] (STJA0011428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/ChIP

Reactivity :

Human

£246.50

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Immunofluorescence analysis of human-liver tissue. 1, Histone H4 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Histone H4 antibody (6-55 aa) (STJ93539)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-HIV-1 gp120/160 antibody [109A2.01] (STJ16102420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF

Reactivity :

Human

£636.50

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Anti-HIV-1 gp120/160 antibody [109A2.01] {Alexa-fluor-647} (STJ16102418)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF

Reactivity :

Human

£770.50

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Anti-HIV-1 gp120/160 antibody [109A2.01] {Biotin} (STJ16102419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF

Reactivity :

Human

£712.50

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Anti-HIV-1 gp120/160 antibody [113B4.07] (STJ16102415)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS/IF/ELISA

Reactivity :

Human

£636.50

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Anti-HIV-1 gp120/160 antibody [113B4.07] {Biotin} (STJ16102414)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/ELISA/FACS

Reactivity :

Human

£712.50

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Anti-HIV-1 gp120/160 antibody [114D3.08] (STJ16102413)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC-F

Reactivity :

Human

£636.50

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Anti-HIV-1 gp120/160 antibody [115F10.05] {Biotin} (STJ16102416)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF

Reactivity :

Human

£712.50

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Anti-HIV-1 gp41 antibody [402D9.14/clone 1] (STJ16102424)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/Neut/ELISA

Reactivity :

Human

£636.50

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Anti-HIV-1 gp41 antibody [402D9.14/clone 1] {Alexa-fluor-488} (STJ16102421)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/Neut/ELISA

Reactivity :

Human

£770.50

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Anti-HIV-1 gp41 antibody [402D9.14/clone 1] {Alexa-fluor-647} (STJ16102422)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/Neut/ELISA

Reactivity :

Human

£770.50

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Anti-HIV-1 gp41 antibody [402D9.14/clone 1] {Biotin} (STJ16102423)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/Neut/ELISA

Reactivity :

Human

£712.50

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Anti-HIV-1 gp41 antibody [417D3.08/clone 2] (STJ16102425)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/Neut/ELISA

Reactivity :

Human

£636.50

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Anti-HIV-1 gp41 antibody [417D3.08/clone 2] {Alexa-fluor-488} (STJ16102430)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FACS/IF/Neut

Reactivity :

Human

£770.50

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