Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using GLDC Rabbit pAb (STJ111974) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GLDC antibody (36-290) (STJ111974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-GLDC antibody (580-749 aa) [SRM] (STJ11107222)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Immunofluorescence analysis of paraffin-embedded Human liver tissue using GLDC Rabbit polyclonal antibody (STJ11105408) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-GLDC antibody (580-749) (STJ11105408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of mouse brain, using GLI1 antibody (STJ116877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GLI1 antibody (350-450) (STJ116877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ73750 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-GLI1 antibody (Internal) (STJ73750)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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Immunofluorescence analysis of Hep G2 cells using GLI2 Rabbit polyclonal antibody (STJ11105567) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GLI2 antibody (327-442) (STJ11105567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of various lysates using GLI2 Rabbit pAb (STJ119206) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-GLI2 antibody (STJ119206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of HepG2 cells, using GLI-3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-GLI3 antibody (11-60 aa) (STJ93279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of HeLa cells, using Gli3 antibody (STJ11103514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Gli3 antibody (1301-1580) [S4MR] (STJ11103514)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Immunofluorescence analysis of A-549 cells using GLO1 antibody (STJ23800). Blue: DAPI for nuclear staining.

Anti-GLO1 antibody (1-184) (STJ23800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-GLP1 antibody [SM0484] (STJA0010484)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€213.13 - €291.88

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Western blot analysis of various lysates using GLP1R Rabbit pAb (STJ111286) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-GLP1R antibody (1-140) (STJ111286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using GLP-1R Polyclonal Antibody

Anti-GLP1R antibody (101-150 aa) (STJ93281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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STJ72860 (1µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-GLP1R antibody (Internal) (STJ72860)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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Anti-GLP2R antibody (180-260 aa) (STJ192649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

€60.00 - €315.00

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Immunofluorescence analysis of C6 cells using GLRX antibody (STJ27268) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GLRX antibody (1-106) (STJ27268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from Jurkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GLRX antibody (8-58 aa) (STJ196533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of SH-SY5Y cells using Glutaminase (GLS) antibody (STJ23808) at dilution of 1:150. Blue: DAPI for nuclear staining.

Anti-GLS antibody (610-669) (STJ23808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of U2OS cells using GLS2 Rabbit polyclonal antibody (STJ118469) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GLS2 antibody (545-602) (STJ118469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemical analysis of paraffin-embedded Pancreatic carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Glucagon antibody (2-100 aa) [PT1696] (STJ196766)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

€217.50 - €315.00

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Immunohistochemistry analysis of paraffin-embedded rat pancreas using Glucagon at dilution of 1:2000

Anti-Glucagon antibody [SM0406] (STJA0010406)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Rat

€213.13 - €291.88

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Anti-Glucagon antibody [SM5032] (STJA0005032)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

€291.88

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Western blot analysis of extracts of various cell lines, using GLUD1 rabbit monoclonal antibody (STJ11102284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GLUD1 antibody (459-558) [S4MR] (STJ11102284)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Anti-GLUD1 antibody (54-242) (STJ29946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using GLUD2 antibody (STJ28687) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GLUD2 antibody (319-558) (STJ28687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ11104567) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GLUL antibody (1-373) (STJ11104567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase (GLUL) Rabbit polyclonal antibody (STJ27390) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GLUL antibody (1-373) (STJ27390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-GLUT1 antibody [SM4989] (STJA0004989)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

€291.88

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Immunohistochemistry of mouse brain with Anti-Glucose Transporter GLUT10 primary antibody (STJ501199) at 1:100 dilution in Buffer.

Anti-GLUT10 antibody (500-536) (STJ501199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-GLUT10 antibody (500-536) {Biotin} (STJ501200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-GLUT10 antibody (500-536) {FITC} (STJ501201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunohistochemistry of rat tissue with Anti-GLUT12 primary antibody (STJ501202) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GLUT12 antibody (C-Term) (STJ501202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/DB/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-GLUT12 antibody (C-Term) {Biotin} (STJ501203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/DB/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-GLUT12 antibody (C-Term) {FITC} (STJ501204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IP/DB/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunohistochemistry of rat kidney with Anti-Glucose Transporter GLUT2 primary antibody (STJ501205) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-GLUT2 antibody (1-50) (STJ501205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€583.13

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Anti-GLUT2 antibody (1-50) {Biotin} (STJ501207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-GLUT2 antibody (1-50) {FITC} (STJ501208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-GLUT2 antibody (473-523) (STJ501206)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

€583.13

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Anti-GLUT2 antibody (473-523) {Biotin} (STJ501209)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

€660.63

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Anti-GLUT2 antibody (473-523) {FITC} (STJ501210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

€660.63

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Anti-GLUT2 antibody (Internal) (STJ73814)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow/Pig

€390.63

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Lane: 1, 4, 7 and 10 - Sal 3 month, Lane: 2, 5, 8 and 11 - Glu 3 month, Lane: 3, 6, 9 and 12 - CB 3 months, Last Lane: MWM (broad range markers). Cortical extracts (10 ug total protein/lane) on 10% SDS-PAGE gel. Primary antibody Glucose transporter type 3 (STJ501211) diluted 1:2000 in buffer. incubation in primary antibody for 170 minutes at RT, washes 3x10 minutes in TBST. Secondary antibody: Alkaline phosphatase conjugated goat anti-rabbit IgG (1:5000)

Anti-GLUT3 antibody (400-493) (STJ501211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€583.13

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Anti-GLUT3 antibody (400-493) {Biotin} (STJ501212)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-GLUT3 antibody (400-493) {FITC} (STJ501213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/FC/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunohistochemistry of rat kidney with Anti-Glucose Transporter GLUT 4 primary antibody (STJ501214) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GLUT4 antibody (450-509) (STJ501214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€583.13

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Anti-GLUT4 antibody (450-509) {Biotin} (STJ501215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-GLUT4 antibody (450-509) {FITC} (STJ501216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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STJ72157 (0. 03µg/ml) staining of Human (A) and Mouse (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Glutamate Dehydrogenase antibody (Internal) (STJ72157)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

€390.63

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