ELISA Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using BTK antibody (STJ114611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BTK antibody (173-272) (STJ114611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using BTK antibody (STJ22844) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-BTK antibody (30-240) (STJ22844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 293 cells using Btk Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-BTK antibody (490-570 aa) (STJ96711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of mouse spleen with Anti-BTK primary antibody (STJ500303) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BTK antibody (550-650) (STJ500303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£400.50

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Anti-BTK antibody (550-650) {Biotin} (STJ500304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£497.50

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Anti-BTK antibody (550-650) {FITC} (STJ500305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Monkey/Mouse/Pig/Rat

£497.50

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Immunofluorescence analysis of mouse spleen using BTK Rabbit monoclonal antibody (STJ11101540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BTK antibody (560-659) [S0MR] (STJ11101540)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis using Btk monoclonal antibody against K562 (1) , MCF-7 (2) , Jurkat (3) and HEK293 (4) cell lysate.

Anti-BTK antibody [7F12H4] (STJ97885)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£149.00 - £360.00

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Anti-BTK antibody [PT0682R] (STJA0039288)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

£150.00 - £540.00

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Western blot analysis of K562 cells using CD272 Polyclonal Antibody

Anti-BTLA antibody (101-150 aa) (STJ92108)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using BTLA Rabbit pAb (STJ110675) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-BTLA antibody (31-241) (STJ110675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using BTLA antibody (STJ115410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BTLA antibody (31-241) (STJ115410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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STJ71778 (1µg/ml) staining of Mouse Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BTLA antibody (C-Term) (STJ71778)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

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Anti-BTLA antibody [DM182] (STJA0038123)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/ELISA

Reactivity :

Human

£432.50

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Anti-BTN1A1 antibody [SAA1669] (STJA0020097)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Western blot analysis of various lysates, using BTN2A1 antibody (STJ11104980) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BTN2A1 antibody (1-100 aa) [S4980RM] (STJ11104980)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of extracts of RD cells, using BTN2A1 antibody (STJ112451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BTN2A1 antibody (30-240) (STJ112451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-BTN2A1 antibody [SAA2136] (STJA0027882)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/FC

Reactivity :

Human

£231.50 - £286.50

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Anti-BTN2A1 antibody [SAA2136] {APC} (STJA0029825)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £352.50

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Anti-BTN2A1 antibody [SAA2136] {FITC} (STJA0028859)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £284.50

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Anti-BTN2A1 antibody [SAA2136] {PerCP} (STJA0030791)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£265.50 - £330.50

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Anti-BTN2A1 antibody [SAA2136] {PE} (STJA0031757)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £352.50

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Anti-BTN2A1/2 antibody [SAA2137] (STJA0027883)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/FC

Reactivity :

Human

£231.50 - £286.50

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Anti-BTN2A1/2 antibody [SAA2137] {APC} (STJA0029826)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £352.50

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Anti-BTN2A1/2 antibody [SAA2137] {FITC} (STJA0028860)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£231.50 - £284.50

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Anti-BTN2A1/2 antibody [SAA2137] {PerCP} (STJA0030792)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£265.50 - £330.50

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Anti-BTN2A1/2 antibody [SAA2137] {PE} (STJA0031758)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£282.50 - £352.50

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Anti-BTN3A1 antibody (STJ192023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-BTN3A1 antibody [DM92] (STJA0038124)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/ELISA

Reactivity :

Human

£432.50

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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200

Anti-BTN3A1/2/3 antibody (41-90 aa) (STJ98764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using BTN3A3 Rabbit polyclonal antibody (STJ116482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BTN3A3 antibody (510-584) (STJ116482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 293T-BTNL2, using BTNL2 rabbit polyclonal antibody (STJ29200) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-BTNL2 antibody (246-455) (STJ29200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-BTNL2/BTL-II antibody (Asp27-Gly453) [1A066] (STJA0021904)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/WB

Reactivity :

Mouse

£197.50 - £275.50

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Anti-BTNL3 antibody [SAA1852] (STJA0020280)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-BTNL8 antibody [SAA1848] (STJA0020276)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Anti-BTNL9 antibody [SAA1828] (STJA0020256)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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Western blot analysis of extracts of Rat testis, using Beta-TrCP/BTRC Rabbit monoclonal antibody antibody (STJ11104611) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BTRC antibody (1-100) [S4611RM] (STJ11104611)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of C6 cells using Beta-TrCP/BTRC Rabbit polyclonal antibody (STJ22845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-BTRC antibody (1-120) (STJ22845)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-BTRC/FWD1 antibody (N-Term) (STJ73836)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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Western blot analysis of lysates from wild type (WT) and BUB1 Rabbit monoclonal antibody knockdown (KD) 293T (KD) cells, using BUB1 Rabbit monoclonal antibody (STJ11105045) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BUB1 antibody (1-150 aa) [S5045RM] (STJ11105045)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-BUB1 antibody (1-300) (STJ11100026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using BUB1 Rabbit polyclonal antibody (STJ22846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-BUB1 antibody (1-300) (STJ22846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BUB1 antibody (251-300 aa) (STJ91912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of the lysates from HUVECcells using BUB1 antibody.

Anti-BUB1 antibody (781-830 aa) (STJ91911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using BUB1 antibody (STJ11105018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BUB1 antibody [S5018RM] (STJ11105018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of NIH-3T3 cells using BUB1B antibody (STJ22847) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-BUB1B antibody (1-260) (STJ22847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using BubR1 Polyclonal Antibody

Anti-BUB1B antibody (341-390 aa) (STJ91914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-BUB1B antibody [SAA1518] (STJA0019946)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£198.50 - £1,170.50

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