ELISA Antibodies | St John's Laboratory

Western blot analysis of various lysates using ALG9 Rabbit polyclonal antibody (STJ119346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ALG9 antibody (439-618) (STJ119346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various lysates, using ALK antibody (STJ11104956) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ALK antibody (1334-1520 aa) [S4956RM] (STJ11104956)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of HeLa cells, using ALK Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ALK antibody (1570-1619 aa) (STJ91560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of Neuro-2a cells using ALK Rabbit polyclonal antibody (STJ116404) at dilution of 1:200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALK antibody (1603-1620) (STJ116404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ALK antibody (Internal) (STJ73813)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC

Reactivity :

Human/Dog/Cow

$421.88

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STJ72632 (0. 03µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Alk-Mouse antibody (Internal) (STJ72632)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse/Rat

$421.88

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Anti-Alkaline Phosphatase (intestinal) antibody [V17.1] (STJ16100829)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC

Reactivity :

Cow/Human

$388.13 - $1,788.08

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Anti-Alkaline Phosphatase antibody [BGN/03/661] (STJ16101899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC-P/WB

Reactivity :

Human

$1,001.03

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Western blot analysis of various lysates, using ALKBH1 Rabbit mAb (STJ11102456) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-ALKBH1 antibody (1-100) [S6MR] (STJ11102456)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of U2OS cells using ALKBH1 Rabbit polyclonal antibody (STJ116014). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALKBH1 antibody (110-389) (STJ116014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various lysates using ALKBH2 Rabbit pAb (STJ28262) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-ALKBH2 antibody (1-261) (STJ28262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALKBH2 antibody (140-220 aa) (STJ190426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from Mouse heart, using ALKBH3 Rabbit polyclonal antibody (STJ28371) at 1:4000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ALKBH3 antibody (1-286) (STJ28371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALKBH3 antibody (110-190 aa) (STJ191787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using ALKBH4 Rabbit pAb (STJ110122) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ALKBH4 antibody (1-170) (STJ110122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunofluorescence analysis of A549 cells using ALKBH4 antibody (STJ114467). Blue: DAPI for nuclear staining.

Anti-ALKBH4 antibody (1-302) (STJ114467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ALKBH4 antibody (STJ191915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ALKBH5 antibody (STJ113284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALKBH5 antibody (245-394) (STJ113284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ALKBH6 Rabbit pAb (STJ112044) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ALKBH6 antibody (97-266) (STJ112044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ALKBH7 antibody (STJ22590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALKBH7 antibody (1-221) (STJ22590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ALKBH7 antibody (10-90 aa) (STJ190427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using ALKBH8 antibody (STJ29222) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ALKBH8 antibody (1-230) (STJ29222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using ALMS1 Polyclonal Antibody

Anti-ALMS1 antibody (1530-1610 aa) (STJ91564)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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STJ70332 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ALMS1 antibody (C-Term) (STJ70332)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF

Reactivity :

Human/Mouse/Rat/Dog/Cow

$421.88

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Western blot analysis of A-431, using ALOX12 antibody (STJ116903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-ALOX12 antibody (483-663) (STJ116903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ALOX12B Rabbit polyclonal antibody (STJ118650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ALOX12B antibody (1-250) (STJ118650)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of PC-12 cells using ALOX15 Rabbit polyclonal antibody (STJ28944) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALOX15 antibody (1-230) (STJ28944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ALOX15 antibody (156-206 aa) (STJ195947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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STJ71782 (0. 2µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ALOX15 antibody (Internal) (STJ71782)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

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Immunofluorescence analysis of U2OS cells using ALOX15B Rabbit polyclonal antibody (STJ28945) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ALOX15B antibody (1-351) (STJ28945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of C6 cells using ALOX5 antibody (STJ22592) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ALOX5 antibody (1-200) (STJ22592)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Confocal imaging of U-2 OS cells using ALOX5 Rabbit monoclonal antibody (STJ11101834, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-ALOX5 antibody (100-200) [S4MR] (STJ11101834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Arachidonate 5 Lipoxygenase Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ALOX5 antibody (246-295 aa) (STJ91392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using ALOXE3 Rabbit pAb (STJ110544) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ALOXE3 antibody (1-280) (STJ110544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-Alpaca-IgG antibody (STJA0010224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Alpaca

$64.80 - $340.20

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Anti-ALPG antibody (10-90 aa) (STJ190382)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Alpha Fetoprotein antibody (50-100) (STJ501651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

$577.13

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Anti-Alpha Fetoprotein antibody (50-100) {Biotin} (STJ501652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

$713.48

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Anti-Alpha Fetoprotein antibody (50-100) {FITC} (STJ501653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse

$713.48

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Confocal imaging of paraffin-embedded Rat heart using alpha Sarcoglycan (SGCA) Rabbit monoclonal antibody (STJ11103115, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-alpha Sarcoglycan antibody (288-387) [S5MR] (STJ11103115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$174.83 - $775.58

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Western blot analysis of extracts of various cell lines, using Alpha SNAP antibody (STJ11102959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Alpha SNAP antibody (201-295) [S9MR] (STJ11102959)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Anti-Alpha Subunit antibody [5501] (STJ400009)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$488.03 - $888.98

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Anti-Alpha Subunit antibody [6601] (STJ400011)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$488.03 - $888.98

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STJ73368 (0. 3µg/ml) staining of albumin-depleted Serum (A) and Plasma (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antichymotrypsin antibody (222-236) (STJ73368)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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STJ73055 (0. 01µg/ml) staining of Albumin-depleted Plasma (A) and Serum (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antitrypsin antibody (230-241) (STJ73055)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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STJ73054 (0. 3µg/ml) staining of Albumin-depleted Human Plasma lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-alpha-1-antitrypsin antibody (Internal) (STJ73054)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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STJ73369 (0. 5µg/ml) staining of Human Albumin-depleted Serum lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-alpha-2-macroglobulin antibody (Internal) (STJ73369)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

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Anti-Alpha-Actinin antibody [CB11] (STJ16100814)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB/ELISA

Reactivity :

Human/Chicken

$766.13

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