||Pep-ELISA, WB, IHC, IF
||FOR RESEARCH USE ONLY (RUO).
||Goat polyclonal antibody anti-alpha-1-antitrypsin (230-241) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence research applications.
||0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
||WB-0.01-0.03µg/mlELISA-antibody detection limit dilution 1:128000.
||Store at-20 on receipt and minimise freeze-thaw cycles.
||Reported variants represent identical protein: NP_001121174.1, NP_001121179.1, NP_001121172.1, NP_001121177.1, NP_001121178.1, NP_001121176.1, NP_001121175.1, NP_000286.3, NP_001121173.1, NP_001002236.1, NP_001002235.1.
| Post Translational Modifications || N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases. |
| Function || Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). |
| Protein Name || Alpha-1-AntitrypsinAlpha-1 Protease InhibitorAlpha-1-AntiproteinaseSerpin A1 Cleaved Into - Short Peptide From AatSpaat |
| Database Links || Reactome: R-HSA-114608Reactome: R-HSA-204005Reactome: R-HSA-381426Reactome: R-HSA-5694530Reactome: R-HSA-6798695Reactome: R-HSA-8957275 |
| Cellular Localisation || SecretedEndoplasmic ReticulumThe S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic ReticulumShort Peptide From Aat: SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Names || Anti-Alpha-1-Antitrypsin antibodyAnti-Alpha-1 Protease Inhibitor antibodyAnti-Alpha-1-Antiproteinase antibodyAnti-Serpin A1 Cleaved Into - Short Peptide From Aat antibodyAnti-Spaat antibodyAnti-SERPINA1 antibodyAnti-AAT antibodyAnti-PI antibodyAnti-PRO0684 antibodyAnti-PRO2209 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance